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Identifying an unknownModerator: BioTeam
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Identifying an unknownHi, I am currently taking my microbiology lab final and as is with most microbiology classes, the final consists of identifying an unknown. In my case, we were all given a different test tube which contains two different bacteria. I was hoping to find some help from you all.
So far, I have done a gram's stain, acid-fast stain and a spore stain. The gram's stain concluded many purple rod-shaped cells, which would lead me to believe that it is gram-positive. The acid-fast stain concluded both pink and purple colors The spore stain did not yield any results. On top of the stains, I have done dishes of a variety of media. MSA: I attached a picture of this to my post, as it had the most growth. In case you can't tell, it is white, flaky, and milky looking. TSA: Yellow colones NA: White colonies EMB: No growth at all The possible bacteria that we can be given are: Bacillus subtillis, Staph aureus, Mycobacterium Smegmatis, Micrococcus Luteus, Proteus Vulgaris, Serratia mercescens, Pseudomonas aeruginosa, Escherichia coli Also, Staph and micrococcus are not a possible combination. I'm fairly certain that one of them is Mycobacterium Smegmatis. I really appreciate the help!
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