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SONICATION OF DNA

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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SONICATION OF DNA

Postby bill1 » Wed Nov 17, 2010 11:19 pm

Heya,

Could you help me with this subject? I need to find out how long do I need to sonicate DNA? any suggestions any publications??

I really appreciate your help :)
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Postby canalon » Thu Nov 18, 2010 1:11 am

Have you tried Google? lots of protocols available for free.
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Postby JackBean » Thu Nov 18, 2010 6:51 am

why do you want to sonicate DNA?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby canalon » Thu Nov 18, 2010 1:56 pm

To shear it in small and relatively constant fragment before say cloning. You can also use a fine needle or a nebulizer. it is cheaper and less sequence specific than restriction enzyme, with break point that should be entirely random, which should give a better chance to get complete coverage for sequence, or to avoid cutting in the middle of your gene of interest.
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Postby bill1 » Wed Nov 24, 2010 7:47 pm

Thank you for all replies. Unfortunately I wasn't able to find as many protocols about sonication as I want:(
If anyone of you has knowledge or links about it I would really appreciate it.
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Postby canalon » Wed Nov 24, 2010 7:54 pm

Things like:
http://www.kreatech.com/Default.aspx?tabid=119
http://www.genome.ou.edu/protocol_book/ ... artII.html

First and 5th link in google for DNA sonication. I fail to see what more you would need.
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