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Improving hybridisation signal by removing cytoplasm

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Improving hybridisation signal by removing cytoplasm

Postby jamesthe3 » Fri Oct 29, 2010 2:54 am

Hello everybody over the years we have had persistent & frustrating problems with cytoplasm and its affect on hybridisation FISH probes. Its become clear to me that pre-treatment to remove cytoplasm from samples is key to enable efficient and successful hybridisation of the probe to the target DNA. Excess cytoplasm can result in samples requiring a longer hybridisation time or complete failure of the probe to hibridise to the chromosomal DNA. We recently purchased a product called Procell Cytoclear and have discovered that it greatly improves preparations and FISH studies by removing cytoplasm around the targest cell.
Hope it helps u as much as it did for us!
Last edited by JackBean on Fri Oct 29, 2010 7:11 am, edited 1 time in total.
Reason: link removed
jamesthe3
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Postby JackBean » Fri Oct 29, 2010 7:13 am

first of all, no need to spam twice. Second, you apparently like many products from your company, don't you?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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