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Help! Convert mg/ml to mg/kgModerator: BioTeam
6 posts • Page 1 of 1
Help! Convert mg/ml to mg/kgOkay, I have nitroglycerin at 5mg/ml (in liquid form).
My mice need a dose of 10mg/kg. I have 8 mice weighing approximately 30g. How do I figure out how to dilute to the proper concentration and volume? I think I need to use C1V1=C2V2, but I don't know how to get the end result. PLEASE HELP!!!
You do not need C1V1=C2V2.
Your mice need 10 mg/kg and weight 0.03 kg, calculate X (in mg) the total amount to inject. You have a 5 mg/ml solution divide x by 5 and you know Y the number of ml you need to inject.
Patrick
Science has proof without any certainty. Creationists have certainty without any proof. (Ashley Montague)
I am still confused....I can use c1v1=c2v2...but I guess what I really need to know is how I get C2?
With a C2 of 1mg/ml, and If I am injecting .3ml for a .03kg mouse, then how did I get to this point? So.... For 8 mice weighing approx 30g each (+a little extra just in case) I was given this information. C1= 5mg/ml V1= .8ml C2= 1mg/ml V2=4 I need to add saline to the nitroglycerin to get the proper concentration, dose and volume. I'm lost...
OK the complete solution for a 30g mouse you need
0.03kg x 10 mg/kg = 0.3mg per mouse so with your initial solution you are suppose to inject 0.06ml or 60µl. If you prepare a 1mg/ml solution, 300µl are necessary
Patrick
Science has proof without any certainty. Creationists have certainty without any proof. (Ashley Montague)
Ok now that totally makes sense! Thank you so much for your help, I know i must seem absolutely retarded. I swear I am not!
One last question. How would I prepare the 1mg/ml solution with the original concentration that i have which is 5mg/ml nitrolgycerin? and I also understand how you got to .3mg per mouse, but how did you convert that to 300microliters per mouse? Because that makes sense, we inject .3ml for a 30g mouse. Thanks you again, i dont know why i cant wrap my head around this
1/ dilute 5 times, or in this case use the c1v1=c2v2
2/ 1mg/ml means that you have 1mg of product in 1ml of solution. if you want 0.3 mg of product, how much solution? PS: brain usage is not optional.
Patrick
Science has proof without any certainty. Creationists have certainty without any proof. (Ashley Montague)
6 posts • Page 1 of 1
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