Biology-Online • View topic - DNA Oligo folding

Join for Free!
122491 members

DNA Oligo folding

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

DNA Oligo folding

Postby Skyrre » Tue Oct 26, 2010 2:50 pm


When I design oligos/primers to use in PCR etc, I usually look at secondary structures and mis-priming etc by using either mfold Tm server (secondary structures within oligo) or mfold 2-state Hybridization server for the primer-bindning.

Do you guys know of a method that can do both at the same time? I want to know which structure that will be the most probable to occur (like hairpin or primer-template hybridization). I know I can look at the Tm and delta-G, but would also like to see the full structure of what's most probable.

Is there such a tool or is it too much to take into consideration when calculating free energies and melting temperatures?

Posts: 5
Joined: Wed Mar 31, 2010 12:31 pm

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 0 guests