Login

Join for Free!
61321 members
Home Forum Dictionary Articles Tutorials Books Directory Share your work


What temperature is needed to destroy RNA?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Post a reply

What temperature is needed to destroy RNA?

Postby unibas » Tue Oct 19, 2010 9:05 am

Hi, does anyone know by any chance what temperature is needed in vivo to destroy mRNA? thanks.
unibas
Garter
Garter
 
Posts: 9
Joined: Tue Aug 09, 2005 7:59 am
Top

Postby JackBean » Tue Oct 19, 2010 9:25 am

in vivo? In vivo is RNA degraded by RNases, not by temperature.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5325
Joined: Mon Sep 14, 2009 7:12 pm
Top

Postby unibas » Tue Oct 19, 2010 10:29 am

yes, I know.
but I can temporarily increase the temperature in a very limited space within a cell, and I want to know what temperature I need to reach in order to destroy mRNA.
unibas
Garter
Garter
 
Posts: 9
Joined: Tue Aug 09, 2005 7:59 am
Top

Postby JackBean » Tue Oct 19, 2010 10:46 am

well, RNA is quite unstable by itself, so like 60-80°C could be fine...
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5325
Joined: Mon Sep 14, 2009 7:12 pm
Top

Postby unibas » Tue Oct 19, 2010 11:05 am

ok good, I'll try that. thanks!
unibas
Garter
Garter
 
Posts: 9
Joined: Tue Aug 09, 2005 7:59 am
Top
Post a reply

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 0 guests