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What temperature is needed to destroy RNA?

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What temperature is needed to destroy RNA?

Postby unibas » Tue Oct 19, 2010 9:05 am

Hi, does anyone know by any chance what temperature is needed in vivo to destroy mRNA? thanks.
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Postby JackBean » Tue Oct 19, 2010 9:25 am

in vivo? In vivo is RNA degraded by RNases, not by temperature.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Postby unibas » Tue Oct 19, 2010 10:29 am

yes, I know.
but I can temporarily increase the temperature in a very limited space within a cell, and I want to know what temperature I need to reach in order to destroy mRNA.
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Postby JackBean » Tue Oct 19, 2010 10:46 am

well, RNA is quite unstable by itself, so like 60-80°C could be fine...
http://www.biolib.cz/en/main/

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Postby unibas » Tue Oct 19, 2010 11:05 am

ok good, I'll try that. thanks!
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