Human Anatomy, Physiology, and Medicine. Anything human!
What is claimed is:
1. A method for molecularly imprinting a material, comprising: forming a solution comprising a solvent and (a) a polymeric material capable of undergoing an addition reaction with a nitrene, (b) a crosslinking agent, (c) a functionalizing monomer, and (d) an imprinting molecule; evaporating the solvent to leave a residue; exposing the residue to an energy source, thereby forming a crosslinked polymeric substrate; and extracting the imprinting molecule from the crosslinked polymeric substrate.
The method according to claim 1, wherein the imprinting molecule is selected from the group consisting of acetaminophen, amilacin, amitriptyline, chloramphenical, cyclosporine, desipramine, digitoxin, digoxin, disopyramide, ethosuximide, flecainide, gentamicin, imipramine, hanamycin, lidocaine, methotrexate, carbamazepine, N-acetylprocainamide, metilmicin, nortriptyline, phenobarbital, phenytoin, procainamide, quinidine, salicylate, streptomycin, theophylline, tobramycin, valproic acid, vancomycin, ethanol, amphetamines, barbiturates, benzodiazepine, buprenorphine, cannabinoids, cocaine, cocaine metabolites, fentanyl, lysergic acid diethylamide, methadone, nicotine, nicotine metabolites, opiates and phencyclidine.
10. The method according to claim 1, wherein the imprinting molecule is selected from the group consisting of acetaminophen, amilacin, amitriptyline, chloramphenical, cyclosporine, desipramine, digitoxin, digoxin, disopyramide, ethosuximide, flecainide, gentamicin, imipramine, hanamycin, lidocaine, methotrexate, carbamazepine, N-acetylprocainamide, metilmicin, nortriptyline, phenobarbital, phenytoin, procainamide, quinidine, salicylate, streptomycin, theophylline, tobramycin, valproic acid, vancomycin, ethanol, amphetamines, barbiturates, benzodiazepine, buprenorphine, cannabinoids, cocaine, cocaine metabolites, fentanyl, lysergic acid diethylamide, methadone, nicotine, nicotine metabolites, opiates and phencyclidine
so please tell me if I'm reading this right.....if we do not use any of these chemicals in the above paragraph, this goes away or it stops?????
Answer please......and hey, why, why, why on acetaminophen? what's wrong w/ that???
you and i both wish you were not reading correctly i have been on this high level military industrial espionage theory regarding panspermia and unfolding galactic issues and a serise of obviously terrestrial subjegated motives for camoflaging biomarker dispersion efforts with scalar galactic helix spiral biomarker we commonly call DNA..... .. . . . . the only agent that could stand witness wouold be the beloved Tardigrade........
and an exxxxxxxxtra sssssssssssssssssssss on your bad dreams
Electroporation Wizard mini-prep kit
(Promega) Stable and transient transfection of cells
DOTAP liposomal transfection protocol
Separation of proteins by polyacrylamide gel
Electrophoresis Preparation of protein probes
EXPERIMENTAL What, then, determines the rate of conversion of cellulose fibers (containing many long chains of cellulose molecules) to individual, shorter chains that are more easily hydrolyzed? This question can be examined from the perspective of the cellulase enzymes themselves and from that of the microorganism responsible for synthesizing the enzyme and utilizing the hydrolytic products. For enzymatic hydrolysis of natural celluloses, several determinants of hydrolysis rate have been proposed, including crystallinity, degree of polymerization, particle size, pore volume, and accessible surface area .
Since cellulose hydrolysis is a surface phenomenon, available surface area is a potential determinant of hydrolytic rate, although there remains some debate about what constitutes the "available" surface area. Several studies have shown that the pore structure of cellulosic materials can accommodate particles of the size of a cellulolytic enzyme (223, 224, 648, 678, 736), and good correlation has been observed between total surface area (estimated from solute exclusion measurements and assumed pore geometries) (647) and the rate of substrate hydrolysis. Gama et al. (202), however, applying a modified solute exclusion technique to five different celluloses, have reported that cellulolytic enzymes do not penetrate the pore structure of purified celluloses. Moreover, these workers point out that effective cellulolysis requires synergism among several enzymes, the combined size of which is larger than the micropores that would accommodate a single enzyme. They conclude that "the external surface area, including the macropores, represents a measure of the effective contact area between cellulose and enzymes in the beginning of the reaction. However, this contact surface is not, in itself relevant to cellulose reactivity...because fragmentation can greatly increase accessible surface area" (202). This is an intriguing interpretation worthy of further study. Unfortunately, a meaningful quantitative relationship between surface area and the kinetics of cellulose hydrolysis did not emerge from their work, in which cellulose disappearance was reported only after a fixed incubation period (6 h). It is likely, however, that pore structure is a much more important determinant of hydrolysis in natural biomass materials than in purified celluloses, which have relatively smooth surfaces and lower porosity. On the whole, the relationship between surface area and hydrolytic rate in cell-free systems is rather equivocal.
By contrast, the relationship between available surface area and rate of cellulose digestion in whole-cell systems is strong across a variety of independent measurement techniques, suggesting that the available surface area is a more important determinant of rate of hydrolysis or utilization than is crystallinity. In mixed ruminal bacteria, where cellulolytic enzymes are retained primarily on the cell surface and the effective size of the catalytic unit is the size of the bacterial cell, it is clear that gross specific surface area (the external surface area excluding micropores) is an effective determinant of hydrolysis rate (416, 733). For F. succinogenes, the rate of succinate production from cellulose is directly proportional to surface area measured by absorption of Congo red dye (416). For mixed ruminal microbes, cellulose removal determined by weight loss is directly proportional to the gross specific surface area of the fiber, calculated from the measured dimensions of cellulose particles (733).
I ask.......... ....could a treatment regimine be initiated by the next paragraph????
Even if hydrolytic cleavage of bonds is moderately rapid, cellulose hydrolysis may be impeded by the inability of enzymes to access additional substrate. This is likely to be due in part to most cellulose chains being buried within the microfibrils. Moreover, coverage of some surface chains by the "footprint" of enzyme molecules (particularly those in a complexed form) that covers many bonds (212) (see "Adsorption" below) physically restricts the binding of additional enzyme molecules to neighboring sites on the fiber. Finally, continued hydrolysis of cellulose requires both excision and removal of hydrolytic products from the site of attack to expose underlying cellulose chains to the enzymes. Walker et al. (718) have made direct measurements of the fragmentation of cellulose (i.e., the breakdown of cellulose into smaller particles, resulting from a separation of associated microfibrils or chains) resulting from the action of T. fusca cellulase and have shown that fragmentation precedes most of the release of reducing sugars. The importance of fragmentation is suggested from comparisons of the rate of weight loss of different cellulose allomorphs by pure cultures of ruminal cellulolytic bacteria and by mixed ruminal microflora (729). Allomorphs with virtually identical unit cell dimensions and similar particle sizes and RCI values showed considerable differences in rate of utilization, as measured by weight loss during bacterial growth. The most slowly utilized allomorphs (cellulose II and cellulose IIIII) were those that are thought to contain—in addition to the intrachain and interchain hydrogen bonds present in all crystalline allomorphs—hydrogen bonds between adjacent sheets, which would impede the fragmentation of microfibrils into individual chains. The relationship between fragmentation and cellulose hydrolysis has not been quantitatively addressed. Fragmentation would be expected to result in an increased reaction rate with time due to increased surface area. However, rates of cellulose hydrolysis by growing bacteria have been found to be constant or to decline with increasing substrate conversion (see "Kinetics of microbial cellulose utilization" below), consistent with the notion that factors other than increased surface area due to fragmentation are the most important in determining hydrolysis rates.
could'nt we just find what amplifies the Q sensing mechinisim in the agent and exploite it by getting the whole macrophage mycoplasm to pay attention to a very loud promoter...... for instance....ingest a certain KNOWN labeled tablet (we need to data mine wayback for ESCHELON sat files find the trensript for the FCCID ref to tagged material RF labled chemical maybe a safe to find a new one and ref sat scans.....one like the reasearch chemical like 2CI.......) and with an external acoustic feild basically HERD the mycoplaslog to one appengage and have an external plate 3.5 voltz 7 hrz gold electrode inside a synthetic LEVEN/agarto induce loud promoter.... like some doped amino acid charged protien gluton lattace at the apendages extreme and HERD off the Spian onto the plate... and
use spent GREEN SALT decontamination protocal for disposal of MYCOPLASMjust a thought............very cold ....
.................................curious................................................ but 7hrtz acoustic that is ..and 3.5V electro .... this coveres electro/kenetic but not photonic .......
any data on UV spectrum or refracture frequency has to be a reason for those fake cloude SOLAR LENZ that have to be more than experiments....
http://www.patentstorm.us/patents/59692 ... ption.html
Thanks so much Nadas! I appreaciate it, really do. I'm going to go back and re-read your post then get off line....long-arse day, you know what I mean?
Okay, this is an official "I'm sorry" for calling anyone a dumdum.....I mean that....but you gotta understand.....this sh it pisses one off.....
I did not deserve to get this....(not like anyone did, but you really got the wrong person with me...)
Now, Nadas and any other intellectuals around......can you, dare you to answer this.....
My research has led me to the gov't and foundations doing this, but I'm really thinking they don't know what's up- I do not say that in a condensending way, but I really think they don't know what is about to hit them....I'm thinking this is over their heads....anyone care to
gimme their opinion here???? I'm just guessing....
Anyway, sorry to all, not even my intent to get big guilty parties involved in trouble either....just wanted to find out what the hell hit me and how....
thinking, catch it early-there may be hope to cure,...HAHAHAHA.....I'm phucked!
Anybody have some heroine??????
I'm looking for a 6months supply!!!!!!! (just joking moderaters!!!)
Well, guess I'm going to start praying asking God to block this laser or this molecular wave coming at me.....yeah, that is my new plans.....
and if whomever can stop this, i will never post again if you can help me....give you a car too......Please??????
Last edited by disinfo on Thu Dec 21, 2006 11:13 am, edited 1 time in total.
and merry phucking christmas to you too......
sticks and stones/////sounds like your arse got into trouble for letting a plane dumdum (myself) figure this out.....gotta love it!
Merry christmas anyway!!!!!!
PS: My research led me to the answer....too bad I can;t do anything about it though.....
Betcha 500.00 right here, right now, that this is porhyrins!!!!!Porhyrin related, as in rings, or metals, or polymers.....ie, porhyrin polymers....
you game???? why, why not???
Hey thanks there disinfo mean one....i got this from your threat at the end!!
I can tell you from experience that this infection has a profound effect upon the human psyche. I’m not qualified to speculate by what process the infection interferes with human neurological function, I can only tell you from experience that it affects a wide range of emotions which closely resemble bi-polar syndrome. When I improved my health, these emotional extremes subsided as well.
I have felt the same anger, fear, and anxiety that London frequently displays. I have seen this in others as well. As such is the case, I am not at all offended by London.
I have been lurking here for quite some time and feel that London is sincerely trying to share his/her ???? knowledge with us. I appeal to you to please try to understand the effects of this infection and be patient with us.
MOST unlike me.....
I read your posts with great interest - they were a breath of fresh air. I suffer from this too and believe me, I understand the reasons for London posting such things. Just once in a while you have to let go. Apologies to London but please think before you post London. Frank we need more people like you. I don't understand this: London is highly intelligient but obviously suffering. I think she has good intentions. Taking everything into consideration I still can't fathom why she lashes out with complete disregard for other people's feelings. Why London? Take a minute to ask yourself. Please don't anyone try and tell me this condition takes over your life, your thoughts and your reasons for being. I know that. And still, taking everything into account, I can not make sense of why you insult people like you do London. That is my point.
I can tell you one thing this disease promotes: FEAR. Fear of the establishment, fear of doctors, fear of the unknown. London, don't take it to heart, don't worry. Be a positive force. DISINFO
To re-iterate and clarify, I am not a biologist nor physician.
I suffered from severe infection for several years until I decided to take every measure to improve my health by breaking some old very bad physical habits, get plenty of rest, and eat right.
After I lost 30 pounds, and started eating right, the fatigue, confusion, mood swings, and lesions improved, but persisted to a lesser degree.
I experienced a severe otic infection of the outer ear canal and sought medical help. The antibiotics and ear drops that the doctor prescribed had no effect upon the infection for 2 weeks. The doctor then prescribed Bausch and Lomb brand Acetic Acid 2% in Aqueous Aluminum Acetate Otic Solution NDC number 24208-615-77 which relieved the pain and weeping fluid in less than 24 hours.
I later applied some of the left over solution to a couple of my lesions and found that it hurt like bloody hell and only made the lesions more inflamed. However, once I stopped applying the solution, the lesions healed completely and did not return.
I did not have enough of the solution to apply to all of my lesions, so I purchased food grade vinegar, and diluted it to 2.5% acetic acid by mixing it with distilled water.
The vinegar also hurt like bloody hell. However I feel that it diminished the infectious agent to a point where my immune system could control it.
Please don’t anyone drink diluted vinegar. I don’t think that it will harm you any more than drinking the juice from a dill pickle jar, but the gastric effect will probably have you cursing my name.
I strongly suspect that the topical application of acetic acid would not have worked if my immune system had not been previously improved to a point that it was able to continue suppressing the infection.
It is also notable that I had been taking anti-biotics for several weeks prior to applying the acetic acid solution, which may have also affected my condition.
Please allow me to state clearly to everyone that I am not making any recommendations on how to proceed with your own state of health. My intent here is only to report to you what measures I took which I strongly feel improved my condition to a more tolerable degree.
year supply heroine wont help its mostlikely on the list.......
lets get it straight this C3 "STRAY" agent is likely subjegated thru "agent green" you know the FUSA blanket covering all illegal crops so not matter your source your stash will be tagged and radio labled with this agent...... and idont care if you got Lebonese amber ..they can still alter the metabolic functions at the "protected" target ...... so that doobie on sunday befor used to calm you but now its got you wired,paranoid, str8 crackheaded....... go go bush doctor homeland security.
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