Human Anatomy, Physiology, and Medicine. Anything human!
and if light is in short supply and you are not upset with me... may i oblige
to partake in the dark arts of diligent humanity by enlightening those striken by those that bottem feed on strife.
yeah .....suppose we connect all the dots ? do we feel better? do we get expelled from the evil?.... .. . . . .........
i bet none of that would ever happen and logic hinting the blueprints for making a dead biologists list...... . . so go find an umbrella.
Nanodosimetry of Low Energy (0.1 - 100 eV) Cation Damage to
L. Sellami, F. Martin, D. Hunting, S. Lacombe, M. A. Huels (Dept. of
Nuclear Medicine and Radiobiology, University of Sherbrooke, QC,
The importance of heavy ions in radiobiology is twofold: (1) they
represent the most efficient and volume selective mode of radiotherapy
of deep-seated and non-operable tumors, (2) in space environments, or
at supersonic altitudes, the most lethal radiation consists of cosmic
rays which have a high efficiency to induce clustered DNA lesions,
mutations, and cancer. Thus, the study of their effects on DNA is
essential for radiation risk assessment, dosimetry, and efficient use
of hadrontherapy. Here, we investigate damage to DNA and its
components, induced by heavy ion impact, via a novel ion-plasma
method, which allows us to probe ion energy depositions in the 0.1-100
eV/nm range in nanoscopic biomolecular films. Cations are generated by
electron impact in ultra pure gases (Ar, N2, CO, etc.), and are
uniformly accelerated by grids towards the inside surface of a
cylinder where an organic film was deposited. After ion irradiation at
a specific energy and ion dose, the film is recovered and analyzed.
For DNA, gel electrophoresis is used to quantify yields of single,
double, and multiple strand breaks. For DNA components
(mononucleotides), fragmentation and new products are measured by HPLC
[R1.073] The Electrophoretic Mobility of Proteins near Surfaces
Perumal Ramasamy (DEPARTMENT OF MATERIALS SCIENCE AND ENGINEERING,
SUNY STONY BROOK UNIVERSITY, NY 11790), Avtar Singh (Stuyvesant High
School), Miriam Rafailovich, JONATHAN SOKOLOV (GARCIA CENTER FOR
POLYMERS AT ENGINEERED SURFACES, DEPT OF MATERAILS SCIENCE AND
ENGINEERING , SUNY STONYBROOK UNIVERSITY, NY 11790)
We have attempted to apply the methods developed for surface DNA
electrophoresis (1) for proteomics. Droplets of FITC stained Abumin,
Poly- L-Lysine, or Casein purchased from Sigma were deposited on glass
cover slips. The droplets were then place in contact with a TBE buffer
solution contained in a cell molded from PDMS. Pt electrodes were
inserted into the cell and a voltage was a applied. The motion of the
protein was then imaged with a Leica Confocal microscope as a function
of buffer concentration, distance from the surface, and applied
voltage. The mobilities were then compared with those of uncharged one
micron florescent Polystyrene beads. References: 1)Henzel WJ, Watanabe
C, Stults JT., ~!0~Protein Identification: The Origins of Peptide Mass
Fingerprinting.~!1~ J. American Society for Mass Spectrometry. 14
(September 2003): 931-942 2)Mathesius U, Imin N, Natera SH, Rolfe BG.,
~!0~Proteomics as a functional genomics tool.~!1~ Methods of Molecular
Biology 236: 395-414.
*Work supported in part by the NSF-MRSEC program
[R1.074] Determination of Fe-CO bond energy in Myoglobin by transient
Raicu V. (Departments of Chemistry and Physics, University of Toronto,
Canada), Walther M. (Physikalisches Institut, Albert-Ludwigs
Universitat Freiburg, Germany), Ogilvie J. P., Phillips R., Miller
R.J.D. (Departments of Chemistry and Physics, University of Toronto,
Various molecular motions relevant to the biological function of a
protein are associated with formation and breaking of chemical bonds.
Heme proteins, such as Myoglobin (Mb), are prototypes of molecules
undergoing structural changes activated by a single bond breaking,
such as CO bond to the heme Fe. Standard textbook value for Fe-CO bond
energy in Mb (W_Fe-CO), determined calorimetrically, is \sim25
kcal/mol. However, first-principle molecular dynamics simulations 
predict a value as high as 35 kcal/mol. We studied the dynamics of
Mb-CO by using transient grating spectroscopy , which is sensitive
to density changes occurring through protein motions or thermal
solvent expansion. The energy of the photons absorbed by the sample is
dissipated through: (a) breaking the Fe-CO bond, (b) protein
relaxation, and (c) thermal effects. We freeze the structural
relaxations by embedding the protein in a glass at room temperature,
and correct for the thermal effects by using an unligated analogue of
Mb ? metMb ? as a reference. We obtained W_Fe-CO = 34 ± 3 kcal/mol,
confirming the theoretical predictions .  C. Rovira and M.
Parinello, Int.J.Quantum Chem., 80 (2000) 1172  G. Dadusc, J.
Ogilvie, U. Marvet, and R.J.D. Miller, Proc.Natl.Acad.Sci. USA 98
[R1.075] Reversible Folding of Lysozyme by a Quasi-static Process: A
First-Order-Like State Transition
Yeh Xu-Cheng, Lin Po-Yen, Chang Chia-Ching (Department of Physics,
National Dong Hwa University, Hualien, Taiwan), Kan Lou-Sing
(Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan)
First-order-like state transition is a novel global reaction model of
protein folding. In order to elucidate the general applicability of
this mechanism, a stepwise thermal equilibrate dialysis process is
used, and a model protein, lysozyme is selected. Within this study,
four stable intermediates and renature lysozyme were obtained and
their secondary structure, particle size distribution, thermal
stability, and oxidation state of disulfide bonds were analyzed by
circular dichroism, dynamic light scattering, differential scanning
calorimetry, and Raman spectra, respectively. According to the
experimental evidences in this study, not only first-order-like state
transition model has been verified, both collapse and sequential
models were observed and verified by an overcritical reaction folding
process. We also found that the glassy state which obtained from
directly folding process can convert into the molten globule state and
this indicated the protein folding under difference reaction path may
follow the same folding mechanism. Namely, the mechanism that is
revealed by overcritical folding intermediates may represent the real
mechanism of protein folding. The helix formed prior to the beta-sheet
might indicate that the protein folding was initiated by local
[R1.076] Motif analysis and correlation functions of amino acids in
nonredundant protein sequences.
Mihaela Sardiu, Yi-Kuo Yu (Physics Department, Florida Atlantic
University and NCBI, NIH)
Amino acid motif plays an important role in protein recognition, and
thus important for the study of protein-protein and protein-DNA
interactions. Using the methods from Statistical Physics, we analyze
the amino acid motifs observed in the non-redundant protein sequence
database. In addition to the exhaustive study of the amino acid motif,
we also study the correlation function between various amino acids in
the primary protein sequences. These correlation functions provide not
only useful information for multiple sequence alignment but also
foundations for the assessment of the statistical significance of the
amino acid motif observed.
[R1.077] Self-assembly and dissociation of collagen monomer:
Temperature and ion effects
Alex Dickson, Lancia Guo, Darren Anderson, Darcy Gentleman, Cynthia
Goh (University of Toronto)
Collagen monomers self-assemble in vitro in a variety of ways to form
morphologically similar aggregates to those formed in vivo. Although
much work has been done on investigating the self-assembly pathway,
little is known about the reversibility of the assembly process. A
better understanding of the assembly and disassembly process will help
us to develop a better picture of the underlying mechanisms by which
collagen associates in vivo. Experimentally, we track collagen
assembly and dissociation through static light scattering while
simultaneously using atomic force microscopy to image the intermediate
collagen structures. We have found that partial dissociation of
fibrils can be caused by lowering the temperature of a solution of
self-assembled collagen and that the extent of dissociation can be
controlled by manipulation of the final solution temperature. We have
also investigated the effect of ion concentration and size on the
assembly process. We will propose an assembly model that relies on
dissolved ions screening like-charged collagen monomers and allowing
self-assembly to occur.
[R1.078] Low Energy Electron Interactions with Uracil: the Energetics
predicted by theory
Xifeng Li, Léon Sanche (Université de Sherbrooke), Michael Sevilla
(Oakland University), PES Collaboration
Low energy electrons (LEEs) induce strand breaks and base damage in
DNA and RNA via fragmentation of molecular bonding. This includes the
formation of hydrogen atoms from N-H and C-H bond dissociations in the
bases thymine, cytosine and uracil, respectively. To better understand
the dissociation of uracil induced by LEEs, we theoretically
characterized the potential energy surfaces (PESs) along the N-H and
C-H bonds of the uracil anion, as well as the energetics involved. The
PESs show that, activation barrier of less than 1 eV exists for the
N1-H dissociation with rather flat PES beyond N-H = ~1.5Å . The PESs
for C5-H and C6-H show larger barriers which increasing monotonically
with bond stretching. All the N-H and C-H bond dissociations are
endothermic; the adiabatic PESs suggest the energy threshold for
formation of hydrogen from N-H and C-H bonds are in the order: 0.78
(N1-H ) < 1.3 (N3-H) < 2.2 (C6-H) < 2.7 eV (C5-H). The H-deleted
uracil radicals (U-yl radical family) are found to have exceptionally
high adiabatic electron affinities, namely 3.46 (N1), 3.8 (N3), 2.35
(C5) and 2.67 eV (C6). During the H bond breaking process of an uracil
transient anion, these electron affinities compensate the extra energy
needed to break the N-H or C-H bonds. This process may therefore
explain the large hydrogen yield found experimentally from uracil upon
attachment of LEEs. Potential applications of this process for the
synthesis of uracil analogs using LEE irradiation are suggested.
[R1.079] Diffusion, Dispersion, and Mobility of Single-stranded DNA in
Polyacrylamide Gel Electrophoresis
Roger Lo, Victor Ugaz (Texas Aamp;M University)
The ability to perform DNA electrophoresis in miniaturized
microfluidic systems has the potential to provide a new generation of
low-cost high-throughput genomic analysis technology. Further progress
toward improving separation performance under these conditions,
however, requires a more detailed understanding of diffusion and
dispersion phenomena in the gel matrix. Unfortunately, it has thus far
proven difficult to obtain extensive measurements of these quantities
due in large part to the lack of a convenient experimental platform.
In this paper, we demonstrate the use of microfabricated gel
electrophoresis devices to measure diffusion, dispersion, and mobility
of single-stranded DNA fragments in crosslinked and uncrosslinked
polyacrylamide gels. The microdevice format allows a complete set of
diffusion and dispersion data to be collected in approximately one
hour, as opposed to experiment times lasting several days using
conventional sequencing equipment. By comparing runs using identical
DNA samples, gel formulations, and operating conditions in both
microfabricated electrophoresis devices and an ALF Express automated
DNA sequencer, we are able to isolate the key factors governing
separation performance in each system. The results of these
experiments are then compared with biased reptation theory to extract
information about the gel structure and predict achievable resolution.
The effects of gel composition and polymerization chemistry are also
[R1.080] NEAR ZERO eV SUBEXCITATION ENERGY ELECTRONS BREAK DNA
Frederic Martin, Zhongli Cai, Pierre Cloutier, Darel Hunting, Leon
Sanche (Research Group in the Radiation Sciences, Faculty of medicine,
University of Sherbrooke, Sherbrooke (QC), Canada, J1H 5N4)
The passage of ionizing radiation through a living cell produces about
4 x 10^4 electrons/MeV, with more than 50% having energies well below
the excitation threshold for water (7-8 eV) (M. Michaud et al,
Physical Review, 44(9), 5623-5627, (1991)). We have previously shown
that 5-20eV electrons cause DNA strand breaks via a resonant process
with a maximum at 10eV (B. Boudaiffa et al, Science 287, 1658-1660,
(2000)). The present results demonstrate that very low energy
electrons in the range of 0 to 5eV cause single strand breaks (SSB) in
DNA. Plasmid DNA is extracted from the host bacteria purified and
resuspended in distilled and deionised water. It is deposited on a
chemically clean tantalum, lyophilised and placed in an UHV chamber
for 24 hours before irradiation. After irradiation, plasmid DNA is
retrieved from the UHV chamber and the samples are dissolved in tris
buffer. The different topological forms of DNA resulting from single
strand break formation are separated by electrophoresis gel, stained
by SYBR Green 1, scanned by laser and quantified with the imageQuant
program. The quantification protocol has been optimized to maximize
both sensitivity and linearity. Two resonant peaks are observed with
maxima at 0,8 eV and 2,2 eV ( 10,5 and 7,5 ssb per 10^3 electrons,
[R1.081] Surface Electrophoresis on Flat and Patterned Conducting
Eli Hoory (SUNY Stony Brook), Saumya Sharma (Ward Melville High
School), Stanley Chiang (Great Neck High School), John Jerome,
Vladimir Samuliov, Jonathan Sokolov, Miriam Rafailovich (SUNY Stony
We studied the electrophoresis on surfaces covered with thin
conducting layers of Aluminum, Nickel and Indium Tin Oxide (ITO). The
purpose was to avoid random charge build-ups that occur on
non-conducting surfaces used in previous studies. The chemical and
physical interactions of the DNA with the surfaces determine the
DNA¡¦s mobility. Laser scanning confocal microscopy was used to follow
molecules of DNA labeled with a fluorescence probe, Ethidium Bromide.
An electric field of 2V/cm was used to induce DNA motion by
electrophoretic means over distances of up to 2mm. Mobilities of
Lambda DNA in TBE buffer (concentration = 10-2 M) were measured for
the three surfaces and the mobilites ranged from 7.5 x 10-5 (cm)2/Vs
to 1.33 x 10-4 (cm)2/Vs, approximately a factor of 3 greater than on
oxide-coated silicon. Measurements on Hind III digest DNA were also
made using both flat and structured (with gratings having periods of 1
and 2 micron) surfaces coated with Aluminum to determine the relative
influence of surface chemistry vs. structural effects. Support from
NSF-MRSEC is gratefullyacknowleged
http://flux.aps.org/meetings/YR04/MAR04 ... S6010.html
http://www.t.u-tokyo.ac.jp/tpage/res/re ... 001_h.html
Nadas..blah blah on the cosmic rays have anything to do with our disease. Skin cancer maybe......but you have not connected anything you have said to this disease.
Sky..man you are very angry and have lost it.
Intellectual property (oximoron for this site) when talking about a disease that we have that we need a cure for..for shame on you..how horrible to even think money when people are suffering....what an ass you are. You have shown your true colors.
During the End Times, Good will battle Evil. Where do you stand?
During the End Times, Good will battle Evil. Where do you stand?
our friend is an elctrician who knows somewhat of what my frirend and i are going through. since finding out by looking up each chemical in
renova one at a time and when they have a phrase chemical we looked up each chemical word of the phrase, he has been given us information on electronics, fiber optics, he told us the green is the ground wire, they
use chemicals to spin rubber, the stretchy elastin that is fibrous and comes from feteus was used in this product, it is how it can streatch to
the sky and can roll back down, isn't rubber used to prevent lightning from striking objects objects like humans, they are able and are impregnating gelatin microbeads, they put magnetics in them these things attached themselves have we can visibly see the metal and the dark things. magnets are black for the most part aren't they and it screws themselves into the skin, scalp,feet, so this multi layer film and we have photos that show it goes from the ceiling to the floor and shooting something so horrible down the tubes it makes, remeber the hydrophilics and hydrophobics can make briges to connect and they used quotom dots and LED lights in this, remember i believe it alloys and alkenes that can act like a battery (and they sure used acids in this) remember the word encapsulate, and they have with no doubt in my mind and if they have microscopes and light measuring equipment and switzerland has the main headquarters for tracking lightwaves, there has to be someone that can help up. this thing is a parasite as you are thinking it is as trust and believe it is mechanical and organic and made from carbons as is the protozoa. the worm like micelles are used to be like a mechanical screw, it screws into you skin and scal one end holding onto the electronics in our body the other end acting like a pole standing straight outward, like an antenna it is spinning like a satellite dish, as my stated years ago in her letters. think about cd discs using organin and inorganic material.
think about all the satellites out there, think about self assembly and what that self assemblies into what, what did they what to create, silicone are microships--microchips can hold thousands of drugs and whatever material needed to complete their mission. we now know negatives of photos show this objects and how it can entwine and stitch in and out of our bodies. we need someone who is an expert and knows this disease exist to exam these photos and negatives and cat scans, one of her cat scans shows she is completely engulfed in an object that constructed itself into a shape of a person the ponytail ligand is so clear in this cat scan
the ponytail acts like a mechanical arm, methane is a drilling chemical
this arm acts like a drill, its claws can open and shut and grab. she is not imagination it is reality and think how fiber optics are used, these these shoot outward making a mechanical spindle of some type. open your minds and your eyes you have to closely look at photos and your negative. photographer said to blow your photos up to like 8x10 take a
magnifying glass and look at the objects on your skin in the photos. remember the cdc stated cat scans show parasites (man made or others)
this was stated and known before morgellons existed and we have that print out. my friends cat scans show more attivity than you could image,
you can see these furry balls (resembling micrbeads to an extent) the eyes of they glow white (the white crystals are used for their eyes no doubt about it like the red are used for the mouths. look up the zipper molecule, it is one they found out alzeheimers have and ms patients.
this mechanical organis zippers itself up and she stated that yerars ago,
wouldn't quotom dots be used as the teeth of the zipper, no matter how far fetch anything goes we have to investigate as she stated in letters they sit in a chairlike structure, we just found out in july about cyclohexane and chair configuration, hair chair boat and twisted boat.
they weren't even clever or ingenious to give them a unique name. got to go to work. take care God bless you (Please work with me on this i will get my electrician to give more inform. i can't say too much too him have to be carefule.
to the post who put the two boys laser article, you have it man do you have. look at his jeans you will see glowing white wiry objects on the blue, not the threads in the jeans, look at your next photo down , in the air you will see circles of objects some glowing white some yellowish with a dark spots in them, they are the objects that are carrying these films
they are carrying the microchips. we have photos of my friend driving,
she was trying to capture on film the white objects that move across her, eyes, wall, hair, clothing, and in front of her car, she captured them on film and so much more, those in that picture are them and they are
in containers, they are glowing white and sprout out on contact with the skin, they have a dark gooey substance and crystals attached to them
mr keith hilley saw them he took samples from her home and then was never heard from again. the technical term for sprouting out i guess is sputtering (branching out in electronics or chemistry) they can collapse and be nano size or macro size now and the cameras can see them, look
at news papers look at magazines and you will see circular glowing white
things in mid air, in peoples hair and clothing, specially crown of the head,
remember the crown point chemicals, the collar bone of neck, eyes, forehead at hairline, as it contacts the skin it starts ethcing (glycol--laser ink dye) and sputtering out whatever is on the mold of the film and "knitting" it onto the skin, remember sol gel, it knits itself, in her letters she said stitched to physicians, remember the organim they want to use in nano tech in the so called future for drug delivery, flagella
the ones that have the propellers. remember they are using microbeads and the hair follicles for drug deliver in japan, kligman worked with the japanese scientients alot, with bombyx silk moth, fireflies, translucent gene of jellyfish to make things glow. isn't makeup about making our faces glosw, bacteriorrhodopsin self illuminates, isn't it about pulling our skin tight and firm, an organism that pulls and spin and illuminates does are of that and it is uses our hair follicles, study the hairs on your arm
with a high power magnifying glass, study the ones in your head. got to go
I'm sorry. I found one article relating to R. Seeberi that I could say was even relevant to the infection I have.
So where is the conection? Can anyone else explain? The only way I can see it fitting in is if perhaps one has a gallbladder problem.
Help me to get this someone.
I think you all need to look into photochemistry and photobacteria more.
I've got proof of that wicked stuff right in front of me.....
Pictures don't lie........
you are right about the shrimp also. this a microorganism that attaches to shrimp eggs that looks exactly like the ones that photograph on my walls, they forgot that everything that exist has a shadow and leaves what they call a "residue" as dr. victor mellu stated five years ago. residue from renova you will need emothional help for what you will be going through and why dermatologist are so fast and quick to diagnosis DOP to get us under minder altering drogs to coverup. there are certain plankton that what biologist state act like a petri dish what are petri dishes used for, even microsoft as a digital bug with logic gates and computerize bugs guess what colors black and white and are encoded.
what lovely words we now have, encapsulate, encode, transcript, plastic out of control, self assembly, zipper molecules, photochemicals, photosensivtice, photo masks they went too far. they are messing with children's lives and there genes and chromosones and their offsprings and
the next generation. Halographis and bacterium and fungus and hybrids.
for the postee who stated we are all on drugs, i don't smoke pot, i don't drink and i don't do drugs and won't let chemicals come near me nor does my friend nor does she drink, she researches, she works and she takes care of her family so don't ever make false accusations not ever because you can be held liable. our statements are made with hard detailed documents. it would matter if she did or i did drugs, tetrahydro was used in this prescription tetradry is cannibais which is marijuana which is a mind adultering drug so they say. it is okay for they to put a controll substance in your medication without your knowledge knowing you would go for a job and it would come back positive for marijuana being in your
system, they also used ethers and anesthetics in this product which is to numb you from feeling the hybrid organisms penetrating into your skin.
so wise up and stop insulting people, if you don't want to help get and go bother somebody else. no one needs your cruelsness and insults and we are deeply offended by your accusations.
The other day, I caught a portion of a program on TV. Dr Francis Collins, director of the National Human Genome Research Institute, a public health research facility funded mostly by the government.
Found an article:
http://www.pbs.org/newshour/extra/featu ... enome.html
The race to crack the code has sparked one of the most heated scientific competitions in history.
The two major "code crackers" are Craig Venter, the president of the private company Celera, and Dr. Francis Collins, director of the National Human Genome Research Institute, a public health research facility funded mostly by the government.
Collins got angry. He criticized Venter for using the groundbreaking research to make a profit by selling it to pharmaceutical companies, and for using research produced by Collin's public research team. Unlike Celera, The Human Genome Project posts its findings for free on the Internet every 24 hours. Since Celera is a private company, it considers the information it discovers to be private property and can sell its findings for money.
I'm wondering if the above is what TT is hinting at? Happened in the Clinton years.
Venter, as London had pointed out, owns a private company and sold info to Big Pharma.
That was the year 2000 (and prior).
I can believe that the "research" that stemmed from the above has something to do with the various emerging diseases- to include FIBERS.
Playing with DNA, that is what it is about.
I'm equally certain that the new products play into this as well.
Case in point- dryer sheets.
A poster on another board stated that dryer sheets cause problems. Don't use them.
This isn't the article that the poster cited, but it will shed light:
Several posters to include msc, are questioning products- rightfully so.
When time permits, MSC, I plan to research that patent you post about.
"When you dine with the devil, bring a long spoon."
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