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Human Anatomy, Physiology, and Medicine. Anything human!

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Postby London » Thu May 18, 2006 2:47 am

and if that was not enough proof , then read this:


Article

Transgenic Research

Publisher: Springer Netherlands
ISSN: 0962-8819 (Paper) 1573-9368 (Online)
DOI: 10.1007/s11248-004-4908-7
Issue: Volume 13, Number 5

Date: October 2004
Pages: 397 - 410

Development of a hybrid delta-endotoxin and its expression in tobacco and cotton for control of a polyphagous pest Spodoptera litura


P. Singh1, Mithilesh Kumar1, C. Chaturvedi1, Dinesh Yadav1 and Rakesh Tuli1

(1) National Botanical Research Institute, Rana Pratap Marg, India


Abstract A hybrid -endotoxin protein was designed against a polyphagous lepidopteran insect pest Spodoptera litura, which is tolerant to most of the known -endotoxins. The hybrid -endotoxin was created by replacing amino acid residues 530–587 in a poorly active natural Cry1Ea protein, with a highly homologous 70 amino acid region of Cry1Ca in domain III. The truncated -endotoxins Cry1Ea, Cry1Ca and the hybrid protein Cry1EC accumulated in Escherichia coli to form inclusion bodies. The solubilised Cry1EC made from E. coli was 4- fold more toxic to the larvae of S. litura than Cry1Ca, the best known -endotoxin against Spodoptera sp. None of the two truncated toxins, solubilised from E. coli caused larval mortality. However, trypsinised Cry1Ca protoxin obtained from E. coli and solubilised from inclusion bodies caused mortality of S. litura with LC50 513 ng/ml semi synthetic diet. A synthetic gene coding for the hybrid$-endotoxin Cry1EC was designed for high level expression in plants, taking into consideration several features found in the highly expressed plant genes. Transgenic, single copy plants of tobacco as well as cotton were developed. The selected lines expressed Cry1EC at 0.1–0.7% of soluble leaf protein. Such plants were completely resistant to S. litura and caused 100% mortality in all stages of larval development. Hence, unlike in E. coli, the hybrid -endotoxin folded into a functionally active conformation in both tobacco and cotton leaves. The truncated Cry1EC expressed in tobacco leaves was about 8-fold more toxic (LC50 58 ng/ml diet) compared to expression in E. coli.

Bacillus thuringiensis - hybrid -endotoxin - insect resistance - protein engineering - Spodoptera control - transgenic cotton
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Postby canalon » Thu May 18, 2006 2:54 am

The following is a snippet I found on E. Coli:


Dear London,

I have worked 5 years with the people that published those results, and indeed handled and worked on the collection described in this paper. Believe me, although the name may seem frightening, those mutators are not going to killl us before long. I could suggest you to read about R. Lenski's experiments and you would see that it takes more than 10000 generations to start obtaining a bacteria close to become a new species.

I suggest you to start reading a lot more about introductory biology before worrying bout snippets that you do not seem to fully understand. Because when I see extract of a book on how to have a good post-doc, I really wonder what you are posting. This guide is probably useful for the like of me in the US, but it is nowhere near to be a guide on how to rule the world (I wish I simply had more money than a freshly hired technician...) or to create any deadly disease. And believe me post-docs work hard and are full of good intentions (sometimes a bit selfish, like to get a real job), and I stilla have to meet any scientist likely to even consider creating pathogenic agents for the pleasure...
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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Postby London » Thu May 18, 2006 2:56 am

and here is more proof of my lesions that are littered by hagfish, sea lamprey and cloned with bt.poisioning.......

Now i KNOW WHY THE DAMN GOVERNMENT DOES NOT WANT TO HELP US AND IT IS SIMPLY BECAUSE OUR GOVERNMENT GAVE US

ANTHRAX!!!

AND TO ADD TO THE ANTHRAX . MAKE SURE YOU DON'T FORGET YOUR METHANE GAS........

hERE'S A DOCUMENT THAT THEY CLONED THE SEA LAPREY, HAGFISH WITH BT POISON.......

http://www.blackwell-synergy.com/doi/pd ... .2580419.x
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Postby canalon » Thu May 18, 2006 3:04 am

Huh? what are you talking about? I read the paper and I really don't see how you come to this conclusion :shocked:
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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Postby RANDY » Thu May 18, 2006 3:22 am

This just came in the mail for me. Thought I would share.
--------------------------------------------------------------------------------


NEWS FOR PORTLAND, OREGON
AND SOUTHERN WASHINGTON
KGW NBC Channel 8, Portland Oregon
Thursday, May 18, 2006 11:00PM

From the producer: We are KGW Northwest Newschannel 8... the NBC affiliate in Portland. Our special report is called: Strange Sickness.. reported by Laural Porter. We will also have a written story on Morgellons on our website-- KGW.COM... and I am working to get this video version of the story put on the web as well.

This is the promo we are running for 24 hours before the story airs:

Imagine feeling bugs crawling on you... And fibers growing out of you! "This is perhaps one of the greatest mysteries that I have ever heard of." Is it really a disease...or simply a delusion? "There is something there. People are killing themselves." Unit 8 In Depth: The Strange Sickness.Tonight at 11 on Newschannel 8...Where the news comes first.

We talked with a former Portland doctor who suffers from Morgellons Disease... also, Lisa Wilson-- the mother of a son who recently took his own life to end the suffering of Morgellons Disease... As well, we will interview Dr. Randy Wymore, Director, Research, Morgellons Research Foundation, Oklahoma State University... and Dr. James Hancy-- who is the head of the obsessive compulsive disorder clinic at Oregon Health Sciences University.

At the end of our TV story-- we will include a link to http://www.morgellons.org... KGW will also mention your website-- on our website.

Our sincere appreciation to KGW NBC 8 for their news coverage on Morgellons Disease. Putting a face to this disease will touch the lives of 1000's in the Portland/Vancouver areas.

Thank you,
Ken Cowles, Director
Media/Public Relations
Morgellons Research Foundation
kencowles@morgellons.org

Carrie
Portland, Oregon
campcurry@msn.com





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Postby befour » Thu May 18, 2006 3:33 am

Randy,

Have you read Southcity's recent post about "How to spot a dis-information specialist? It's very informative....... and I believe YOU fit the criteria perfectly!!

You are so predictible!! We can always expect your tantrums when either Cilla or Tam Tam post. What's the matter....are they getting TOO CLOSE to revealing the true cause of this hideous disease.....and the persons' responsible????

Your cover is blown sweetie....don't waste your time being outraged and claiming that you are for real.....give us all a big break and leave us alone!!

You've had your fun and you probably have been paid handsomely for your work, disrupting our forum.

What a horrible thing for a person to do....pretend that they have this disease, for money!!!! Remember the saying "Pay-Back is a Bitch!!!"

befour



southcity wrote:This topic has been overlooked and not discussed for far too long.

How to spot a dis-information specialist.

All who are infected with this illness and have been watching all the discussions concerning it are aware of some items. These I will discuss here today. First of all there are many interest groups set up to defend this issue. They have done so primarily by enforcing/ promoting the delusions of parasites theory. We know this as fact but have you considered exactly how they have and continue to promote this?

First of all, many of the people who are active in this discussion do not have this illness(though many say they do). They are paid by various interest groups to discredit the illness and also those whom have it. It is their job to monitor the different discussion groups and sabotage any real organization or logical posts made by anyone. this happens with a number of controversial topics, including this one. they are not unique to this illnesses discussions. That said lets look at some of the ways they do this.

Ways to spot this type of behaviour
1. Use derogatory language and fly off the handle.

2. they will call others names and make insinuating remarks and otherwise say anything to discredit any valid points being made concerning the topic. they will question others character often.

3. They will most often have the largest number of posts of any of the sites other members. Often by 10 to 20 times as many posts as others making valid points in each particular discussion.

4. Often they will be the very first person to reply after any thought provoking post concerning the topic is made. NOTE: they will often throw out a string of unrelated information and links pointing in many different directions to confuse the point previously made.

5. they present an overabundance of information and avenues of direction to follow up on for those reading/researching. Most information upon looking further into actually has NOTHING to do with what is being discussed. This also is meant to confuse an issue and also serves to make all involved to appear mental or conspiratorial. THIS ALSO SERVES IN MAKING OTHERS LOSE INTEREST IN TOPIC simply because of extremely long discussion threads with an enourmous amount of unrelated information. In our case concerning this illness, I have been told by a dozen or more people that some threads are avoided simply by seeing particular people having been an active participant in the discussion. They annoy people to the point of losing interest, no matter how interesting a topic may be.


6. They claim to have the answer constantly, but each time the answer is different from the any other previously made. Look for the phrases “This is it” or “like I have said before” “I know this is involved” esp. when topic is known to still be a mystery. They will say anything to confuse the reader and get them off track. they are extremely non consistent in thier beliefs.

7. they are non focused and each day will present new and “important information” which is most often non related to their other important info posted previously.

8. often remarks made by them out of frustration is then censored and deleted from discussion.

9. they are intelligent people, but because of the constant posting and monitoring of the discussions, appear to be unemployed. Note: simply tract the times of which their posts are made. Most people will post around the same time of day because of work, family and other responsibilities and obligations.


Ok I think I have made my point. This is not a complete list of methods involved by these people, not by a long shot. But you hopefully get the point and can now start to see through the smoke screen they are responsible for. Do not doubt these people exist. They do and are very active in all types of debatable topics. Trust your gut feelings as they are most often quite correct.
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Postby London » Thu May 18, 2006 4:10 am

Canalon,

I know I do not know much at all about this and the articles are very
confusing. I'm glad that you wrote and wish you all would do so more.

Let me ask you a question. Do you see Bt. as being the same thing as
anthrax? I do and it seems many scientist will agree with that as well, but I would like to hear what you think about that.
_____________________________________

To All:


Effect of electrical charges and fields on injury and viability of airborne bacteria.

Mainelis G, Gorny RL, Reponen T, Trunov M, Grinshpun SA, Baron P, Yadav J, Willeke K.

Aerosol Research and Exposure Assessment Laboratory, Department of Environmental Health, University of Cincinnati, Cincinnati, Ohio, USA. mainelis@envsci.rutgers.edu

In this study, the effects of the electric charges and fields on the viability of airborne microorganisms were investigated. The electric charges of different magnitude and polarity were imparted on airborne microbial cells by a means of induction charging. The airborne microorganisms carrying different electric charge levels were then extracted by an electric mobility analyzer and collected using a microbial sampler. It was found that the viability of Pseudomonas fluorescens bacteria, used as a model for sensitive bacteria, carrying a net charge from 4100 negative to 30 positive elementary charges ranged between 40% and 60%; the viability of the cells carrying >2700 positive charges was below 1.5%. In contrast, the viability of the stress-resistant spores of Bacillus subtilis var. niger (used as simulant of anthrax-causing Bacillus anthracis spores when testing bioaerosol sensors in various studies), was not affected by the amount of electric charges on the spores. Because bacterial cells depend on their membrane potential for basic metabolic activities, drastic changes occurring in the membrane potential during aerosolization and the local electric fields induced by the imposed charges appeared to affect the sensitive cells' viability. These findings facilitate applications of electric charging for environmental control purposes involving sterilization of bacterial cells by imposing high electric charges on them. The findings from this study can also be used in the development of new bioaerosol sampling methods based on electrostatic principles. Copyright 2002 Wiley Periodicals, Inc.

PMID: 12115440 [PubMed - indexed for MEDLINE]
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Wrong

Postby RANDY » Thu May 18, 2006 5:03 pm

Befour:

Let me say right here for the record that you are totally wrong. I have listed my phone number, my address and my first and last name. Hundreds of people have called me on the phone. I have this disease and I know what works and does not.

For you to say that about me shows that you are so off track that I feel very sorry for you.

You can call me any time and talk to me. 434-974-7128 andi fyou do a reversal on the number you can get my home address.
You can contact my personal e-mail. cisfl2004@netzero.com

Where is your personal information? So who is doing what?

I have the correct information. You are WRONG here. VERY VERY wrong.

I will fly off the handle when BS-ers start BS-ing. I have a right to protect others like myself. We just had another suicide. false hope and disappointment cuases them time and time again..

You could not be further from the truth.

I speak nothing but truth and I give out correct information and nayone that knows and has talked to me will tell you so.

You are wrong friend. Really wrong.

Randy

Call me.....I have NOTHING to hide
.
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funny

Postby RANDY » Thu May 18, 2006 5:09 pm

The fact that South wrote that just shows that he is trying to squeeze out the good honest people so that all you others can do your red herring thing.

Those out there. Use your brains. You can see what is going on here.

My phone number is real. Where is Souths number? Where is Befours number? Where is Tams number ..where does he live?..where is Cilla's number?..where does she live? My number and my address is there for all to see cuz this disease is bigger than I am. So I will give out all my info even if it places me at risk.

Also darling I own a business http://translatethisdocument.com and I work a full time job. So please get your facts straight.

Where do all these other people even live? What country..or are they government workers throwing in red herrings????????

What are their phone numbers and real e-mail addresses. Has anyone ever talked to them on the phone? I have spoken to hundreds of people.

And the info about the NIH...ALL TRUE. Call them and check it out.

Who is giving out dis-information? Who wrote that to FIT me...hahahahahha!!!

That is so funny. Anyone with a brain will see what you are trying to do.

So Sophmorish! Honestly. What are we in High School now?

Who are you Befour? That is my question since you are trying to make me look bad.
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Re: Wrong

Postby standby » Thu May 18, 2006 9:40 pm

[quote="RANDY]

We just had another suicide. false hope and disappointment cuases them time and time again..


I speak nothing but truth and I give out correct information and nayone that knows and has talked to me will tell you so.


Randy

[/quote]
Please do not use the deaths of these poor souls as a means to disprove or disrespect others who are actually trying to help the cause and prevent more of that very thing.

You speak of openness and truthfulness.
Putting all ones private info out for all to see may be fearless ...... but truthfull it does not make. No one is obligated to divulge personal info and they are being smart by not doing so. Why continue to entice people to do this?

Your own website is full of disinformation, untruths and little hope for sufferers .............. so be careful where you point that finger.
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False INFO?

Postby RANDY » Thu May 18, 2006 10:01 pm

First of all my site is not filed with untruths. If so, straighten me out and I will correct them with facts. Give me some solid facts and I will change my site..but I guess you will nto do that either..am I correct?

Second..I am not using anyones death for my own advantage. In fact I am doing some good between two people...and am sensative to the reasons why they did what they did....... so you really need to know what you are talking about where that is concerned. False hope kills.

Third, I never forced anyone to give out anything. But I do and will continue to since I know that people need a phone to phone support group and not just one with faceless, voice-less individuals who you have no idea who they really are or where they come from or what they are really up to.

I do not call it fearless. I call it being part of a group that needs more than a computer for support.

From posting my number I received some phone call today and talked for hours to some sufferers....so doing some good is where I am at. I know how one can suffer. I knwo how one can get relief and I know what course this thing has taken since I have been part of it for a very long time.

If you called me you would know that too.

So please...know of what you speak when it comes to dis-in.

Randy
During the End Times, Good will battle Evil. Where do you stand?
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Postby RANDY » Thu May 18, 2006 10:02 pm

Lastly..this is not a pissing contest for me. If it is for you ..I can not help that. So do not take it ever that this is what I am doing. I am not. If you feel I am perhaps you actually are?
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