Human Anatomy, Physiology, and Medicine. Anything human!
Go ahead anyone...explain to me in simple English what this post means..such filler-busting! Such disinformation! Such a waste of a good site!
Anyone..explain the meaning of this post in simple laymans terms. I dare ya! Tam..how about you!?
The most conspicuous internal structures of the endospore of Rhinosporidium seeberi are the 10–16 spherical, 1.0–1.5- µ m bodies that have been termed electron-dense bodies (EDB) or lipid bodies (LB); some authors have regarded them as nutritive stores of lipid or protein while others have regarded them as DNA-containing, ultimate generative units of R. seeberi . The literature is reviewed as supporting either view. We report, for the first time, (i) reactions of the endospores with the salt MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) and (ii) ultrastructural appearances; and suggest that both views on the nature of spherical bodies are valid (i.e. the endospore contains both EDB, and lipid or protein bodies). Well-marked reduction of the MTT with the production of deep-purple staining was seen in a proportion of the spherical bodies, probably the EDB, suggesting that they are actively metabolizing, viable elements with dehydrogenase activity, and that these bodies are the thick-walled electron-dense bodies described as EDB and visualized in the transmission electron microphotograph illustrated in this paper. The spherical bodies showed fluorescent labeling with acridine orange and with ethidium bromide supporting the idea that they contained nucleic acids. TMRE (tetramethyl rhodamine ethyl ester), a mitochondrion-specific dye, also labeled the intra-endosporial spherical bodies. Other bodies (LB) of a similar size that were MTT-non-reducing, electron lucent, and have no organized structure, are probably the lipid or protein containing, inert, nutritive storage bodies suggested by previous authors.
How does this relate..those out there need to know what the great minds on this site think they can comprehend.
Again..my number is 434-974-7128 if you want to really help... call or write me. 434-980-2757 email@example.com
Get your state on board.
Happy Holidays to all except the anti-semites that rule this site.
Check back a few posts ..one guy called me a Jewish female an inbred.
That is who is typing..Nazi's and their friends.
nazi parade in stores or any open air parameter on north american soil.......what the FFFck more do you need to know....
im starting to think you dont even share this illness.... . just go wash you blankets in drano an dry in you oven ??
Biomimetic filaments: Worm-like micelles of block copolymers
Dennis E. Discher, Dept. Chemical & Biomolecular Eng'g, University of Pennsylvania, Philadelphia, PA 19104, firstname.lastname@example.org
Giant and stable worm-like micelles formed in water from a suitably-proportioned series of polyethyleneoxide-based (PEO) diblock copolymer amphiphiles are shown to mimic the wide ranging flexibility of natural filaments from linear phages to microtubules. Worm diameter (d) is found, by cryo-TEM, to scale with the length of the hydrophobic chain (N) of the copolymer as N^0.61, which is close to the strong segregation limit scaling. By fluorescence video imaging of worm dynamics, we also show that the persistence length (lp) of worm-like micelles scales as~ d^2.8, consistent with a fluid aggregate (~d^3) rather than a solid rod (~d^4). By polymerizing the unsaturated bonds of assembled copolymers, fluid worms are converted to solid-core worms, extending the bending rigidity to millimeters. Through partial crosslinking, polymerized worms are shown to lock in a spontaneous curvature at a novel fluid-to-solid percolation point. The dynamics of distinct, branched conformations are also imaged for recently discovered Y-junctioned worm-like micelles composed of diblocks of high molecular weight (>10-15 kg/mol). Finally, block copolymers of hydrophilic weight fraction (wEO) close to the transition between a vesicle- and worm-former are shown to assemble into both structures, allowing encapsulation of worm-like micelles in giant vesicles reminiscent of cytoskeletal filaments enclosed within cells.
Anomalous migration of nucleic acid fragments in gels
Ion-channel molecules with ion-gating properties play an important role in biological membranes allowing for controllable transport of species across cell membranes. We have studied the behavior of such molecules in the adsorbed state on solid electrodes, either by incorporating them into a supported lipid bilayer film (BLM), e.g. gramicidin, or by self-assembling them directly on a gold substrate electrode. In the latter group, glutathione SAM was found to show remarkable gating properties toward redox probe ions (ferricyanide) and metal ions (Cu2+, Ni2+, Pb2+). In the present study, we focused on modeling conformational transformations of glutathione molecules adsorbed on Au and tuning their ion-gating properties by controlling the electric field, local pH, and interacting with glutathione, metal ions. The behavior of glutathione is compared to aliphatic carboxymercaptans. The measurements were performed using the EQCN technique, allowing for fast determination of adsorption transients and interactions of the adsorbate with solution species.
COLL 548 : Protection and deprotection of liposomes by electrostatically anchored PEG chains
R. K. Prud'homme, Princeton U, Dept. of Chemical Engineering, Princeton, NJ 08544-5263, Fax: (609) 258-0211, email@example.com, and Debra Auguste, Department of Chemical Engineering, Princeton University
The reversible association of electrostatically-modified PEG for endocytotic delivery of condensed DNA for gene therapy is presented. The major advantage of liposomal delivery is the fact that it separates the various functions required for successful gene delivery and therefore allows more flexibility than alternate strategies. Two types of polymers are being investigated: comb-graft polymers, where a PEG chain and an anchor is repeated, and single-tailed polymers, where multiple binding sites occur in series followed by a PEG chain. Our strategy is to increase the association of PEG to liposomes by incorporating multiple, relatively weak anchors to produce strong cooperative binding, but to be able to disassociate the PEG polymer from the liposome surface by a pH shift from pH 7.4 in the circulatory system to pH 5.5 in the endosome. Liposomes with varying electrostatic composition are prepared from mixtures of negatively charged DOPG, positively charged DOTAP, zwitterionic DOPC, and DODAP (which has a pKa of 6.7 and can change from neutral to positive under acidic conditions). The electrostatically bound PEG chains can be released from the liposome surface by the pH shift. This permits liposomal fusion in the endosome and release of the DNA contents.
Hierarchical biomolecular self-assembly through electrostatic interactions
Gerard C. L. Wong, Materials Science & Engineering Dept. and Physics Dept, University of Illinois at Urbana-Champaign, 1304 W. Green St., Urbana, IL 61801, Fax: 217 333 2736, firstname.lastname@example.org
We describe the structure and interactions of a novel class of biomolecular self-assembly, in which charged macroions of varying complexity are collectively organized through the interplay of electrostatics and entropy. These interactions are quite general, since all nucleic acids, all cell membranes, as well as most proteins and sugars are charged. The macroions which self-assemble are frequently oppositely-charged, but in contrast to intuition, they can be like-charged as well. We will focus the discussion specifically on our recent work on interactions between charged biopolymers and charged membranes. Early examples include colloidal complexes of anionic DNA and cationic lipids for non-viral gene therapy. We have examined the ‘design rules’ of such complexes by investigating other forms of anionic biopolymer-cationic membrane assembly comprised of biopolymers of different charge, flexibility, and radii. We will also describe novel mesophases of complexes formed from anionic biopolymers and anionic membranes, mediated through multivalent ions. Potential applications of these self-assembled systems outside of gene therapy, from nanofabrication to cystic fibrosis, will be discussed.
COLL 2 : Predicted and observed effects of excluded volume on the self-assembly of protein fibers and other complexes in crowded solutions
Allen P Minton, NIDDK, National Institute of Health, Bethesda, MD 20892-0830, email@example.com
Volume exclusion arising from steric repulsion provides an important driving force for macromolecular compaction and association in highly volume-occupied or 'crowded' solutions. The presence of inert bystander macromolecules at concentrations comparable to those found in biological fluid media may increase rate and equilibrium constants for self-association of a dilute reactive species by one or more orders of magnitude relative to values measured in solutions lacking the bystander macromolecules. We review recent theoretical models of excluded volume effects on the rates and equilibria of protein fiber formation, and the results of recent experimental studies of the effect of volume exclusion on the rate of amyloid fiber formation and the rate of formation of tubulin ring arrays.
COLL 3 : Seeing macromolecular assemblies suitable for drug and gene delivery
Brigitte Papahadjopoulos-Sternberg, Dental School, Microbiology Department, Nanoanalytical Laboratory & University of the Pacific, 3951 Sacramento Street, San Francisco, CA 94118, Fax: 415-831-2813, firstname.lastname@example.org
The potency of drug/gene-loaded carriers is frequently depending upon their morphology adopted in a biological relevant environment. Freeze-fracture electron microscopy is not only a powerful technique to characterize drug/gene carrier at nanometer resolution scale but also the method of choice to study their fate related to drug/gene load, application milieu, and during interaction with cells[1-3]. Using freeze-fracture electron microscopy we studied the morphology of a wide variety of drug and gene carriers such as depofoam particles, cochleate cylinder, liposomes, niosomes, micelles, and cationic liposome/DNA complexes. Furthermore we correlated the morphology of CLDC to their transfection activity under in vitro as well as in vivo conditions. While MLV display a multitude of bilayers and diameters of several micrometers, SUV have only one bilayer and can be as small as 15 nm[1-4]. Because of their small size (5-50 nm), spherical micelles accumulate in pathological areas and are excellent carrier for poorly water soluble drugs . Niosomes are able to adopt geodetic sphere structure. Cochleate cylinders are made of negatively charged lipids and form cigar-like cylinders several tens of micrometers long[1,2]. DepoFoam particles are prepared by double emulsification processes and display chambered inner volumes several tens of micrometers large. Depending upon helper lipid, ionic strength, and gene component CLDC adopt polymorph structures such as spaghetti/meatballs, map-pins, as well as honeycomb structures [7,8]. Parallel studies of transfection activity and morphology of CLDC revealed a fundamental difference between in vitro and in vivo transfection activity. Lipid precipitates displaying honeycomb structure are associated with high transfection rates under in vitro conditions. In vivo transfection activity seems to be associated with small complexes such as map-pin structure
hell with it .............
english is english plain to you plane to me.......
Chrompophore functionalized metal nanoparticles
Inorganic-organic hybrid structures provide a new wat to tailor the properties of next generation nanodevices. Gold nanoparticles and nanorods have now been functionalized with pyrene, fullerene and porphyrin derivatives. Excited state interactions with gold naoparticles have been probed using transient absorption and emission spectroscopy. The emissive properties of surface bound chromphores have been modulated by externally applied electrochemical bias. Application of these organic-inorganic hybrid systems in light energy conversion systems will be presented.
http://www.nano-biology.net/showcitatio ... microscopi
Blah Blah Blah, Blah, Blah, Blah Blah.....nothing to do with what we have.....more dis-info..if not,,please explain to us dummies how this relates in plain English...cuz I looked it up Tam and like your video it has nothing to do with our disease..just another red herring.
WARNING...This disease, which is comprosed of nano particles as fibers in your skin, made of metals, causes the infected one to sit by their computer for hours and hours and type, cutting and pasting and cutting and pasting useless information with the belief system that they are actually making sense!!!
Please note..If you have this step away from your computer or you will never get better..you are being pulled into the computer via your nano particles..step away..also do not use cell phones and your magnetic keys to any hotel or motel room will most likely be erased.
Warning Will Robinson Warning Will Robinson!
If you have this disease, please be aware that Randy, who claims to be helping, has been kicked out of all sites that relate to this illness.
Randy, wants all your medical information and will go out of her way to ask you for it.
Randy, was working at one point for MRF, but then suddenly started putting them down as stupid idiots, as she does here, to those who are trying to put it all together because not one freakin professional will break the protocol that was set up to make sure your disease is never uncovered. I think that she is part of that team!!!!!
If you want to get help from Randy, Go for it. Where are ALL the people that she has helped? What have they for themselves? Gold Bond Powder? Randy has her mind made up about the right way to go about this. Why don't you list your talking points, Randy?
Huuuuummmmm, think maybe one of the reasons you are exceedingly hostile and overly critical is because people don't make those connections for YOU, therefore you can't steal their ball and run with it?
Has the jewish princess always expected things to be handed to her on a silver platter?
What ball are you talking about? Not a single person including you has any idea and I have consulted with people smarter than myself. All you are doing is game playing. So see-thur obvious.
So what you are saying is that you know but you are not telling even those who watch this site and are sick. Those that and call me and tell me that it is a useless site with psycho babble.
I see you want to make money off of sick people so you are not telling..this is such an excuse for not knowing.
Why would you not tell....like Tam talking cryptic..WHY? Cause this is a game for you. Nothing more, nothing less.
YOu have no clue and cover your arse with IM NOT GONNA TELL YOU!
That last statement proves my point and also proves the anti-semite point I made in a previous post.
You are jealous and childish. And you have no idea what you are talking about so stop trying to fake it and make like you are making sense..I understand all the posts..they, like the video are bogus and nothing but disinformation and have nothing to do with our disease..so pretend you have a secret you are not telling.
That is what a public forum is for...right?
Your game is so up. Plus TAM never had any meeting with anyone and never did any TV shows like he said he was going to do and never met with any NEWS people or with Marc from Germany so Marc could go on TV for him to tell the world. All lies and BS. Lies and BS.
The only person you are fooling with IM NOT GONNS TELL YOU NAH NAH NHA NHA NAH is you.
I do hope yo uunderstand that much!
why in the hell would TAM come to this contaminated wasteland
im sure he cant afford the bodyguard service it would take to keep you leeches off ......i have tried very hard to over look your imput into this randy.... and at this point i have no english to put what it is i feel for you and your employer...... god damn....
is there not a single crumb of partched earth for FREE thinkers ........
OK I will stoop to your federal gangsterass tactics......
consinder this strike ONE.... keep fu$%kinig with our donated effort.....
ill proxy off the mapp and shut your fuc&*ing UNKNOWN SKIN site down!!
Bottel neck that bitch like a microbiologist on the river bottom............
I could give a f**k if you think you got my IP cause I got peeps that run ISPs
and a few "human errors" would do your evil trip a world of good....
-off topic NADAS
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