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nuclear acid probe labeling

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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nuclear acid probe labeling

Postby seahow » Sat Sep 25, 2010 7:49 am

I am now preparing run-on assay,and I want some help about it. Dose anyone know it,and tell me some tips about it.

I donnot want label the probe with Isotope(radiolabeled). can anyone tell me the non-isotopel abeling methods . I hope it is sensitive,and popular accepted .

Thanks a lot!
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Postby JackBean » Sat Sep 25, 2010 3:08 pm

you can always use some fluorescent labels like Cy3 or Cy5
http://www.biolib.cz/en/main/

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Postby canalon » Sat Sep 25, 2010 6:16 pm

The DIG system by roche was the first one, there are plenty other available, but I have had good experience with them so link:
https://www.roche-applied-science.com/sis/lad/index.jsp
and the manual that is available was generally a very good information for all Southern and Northern blot related thing (even if you use other products)
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Re:

Postby seahow » Sat Sep 25, 2010 7:42 pm

JackBean wrote:you can always use some fluorescent labels like Cy3 or Cy5


can run-on assay can be proformed by fluorescent labels like Cy3 or Cy5.
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