nuclear acid probe labeling

[seahow]

I am now preparing run-on assay,and I want some help about it. Dose anyone know it,and tell me some tips about it.

I donnot want label the probe with Isotope(radiolabeled). can anyone tell me the non-isotopel abeling methods . I hope it is sensitive,and popular accepted .

Thanks a lot!

[JackBean]

you can always use some fluorescent labels like Cy3 or Cy5

[canalon]

The DIG system by roche was the first one, there are plenty other available, but I have had good experience with them so link:
https://www.roche-applied-science.com/sis/lad/index.jsp
and the manual that is available was generally a very good information for all Southern and Northern blot related thing (even if you use other products)

[seahow]

you can always use some fluorescent labels like Cy3 or Cy5

can run-on assay can be proformed by fluorescent labels like Cy3 or Cy5.