Login

Join for Free!
116894 members


what is a tissuespecific gene? transcription factors?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

what is a tissuespecific gene? transcription factors?

Postby naejjiv » Mon Aug 23, 2010 3:50 pm

afte reading a bunch of papers i am getting more confused than ever.

tissue specificity is the level of expression of a gene in a certain type of cell, am i correct?
so a tissue-specific gene will only be expressed in that type of cell?
how do you decide if a gene is tissue specific? in my current work, i have a gene which is known to be tissue specific from previous researchers' work... when silenced, two organs completely do not develop at all. but these two organs are made from different tissue -- one forms from mesoderm, the other from ectoderm. so how is this gene considered tissue specific?

also, transcription factors are needed for the regulation of gene expression (for binding rna polymerase to dna), yes?
and transcription factors are proteins
proteins are a product of gene expression
so how did these genes which made the protein (TF) get expressed?

please advise.
thank you very much!
naejjiv
Garter
Garter
 
Posts: 1
Joined: Mon Aug 23, 2010 3:42 pm

Re: what is a tissuespecific gene? transcription factors?

Postby canalon » Mon Aug 23, 2010 10:24 pm

naejjiv wrote:also, transcription factors are needed for the regulation of gene expression (for binding rna polymerase to dna), yes?
and transcription factors are proteins
proteins are a product of gene expression
so how did these genes which made the protein (TF) get expressed?


Think how cells are made.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
User avatar
canalon
Inland Taipan
Inland Taipan
 
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Re: what is a tissuespecific gene? transcription factors?

Postby zerhos » Tue Aug 24, 2010 11:00 am

naejjiv wrote:afte reading a bunch of papers i am getting more confused than ever.


........how do you decide if a gene is tissue specific?.............

a


you need to analyze the expression pattern by in situ-hybridizzation
or PCR,Q-PCR on cell lysate of each tissue

also, transcription factors are needed for the regulation of gene expression (for binding rna polymerase to dna), yes?
and transcription factors are proteins
proteins are a product of gene expression
so how did these genes which made the protein (TF) get expressed?


usually by external factors such as hormones, cytokines, etc.
each cell type will respond differently to the same external factor, through different receptors and activate different transcription pathways and therefore different tf
That was in addition to the action of epigenetics
zerhos
Garter
Garter
 
Posts: 2
Joined: Sat Apr 10, 2010 1:25 pm


Postby Martin11 » Fri Aug 27, 2010 8:20 am

Without knowing the gene you are referring to, perhaps the gene product has two distinct functions at two different points in time possibly due to combinatorial effects by different cellular proteins at different stages of cell differentiation (my guess). So I guess zerhos's suggestion has some merit in terms of going about elucidating such interactions. But I think some sort of high throughput gene expression profiling should come first because that would let you see patterns of gene activity and possibly provide specific leads to investigate.
I also agree with zerho about the external factors directing early protein expression in cells
Martin11
Garter
Garter
 
Posts: 3
Joined: Fri Aug 27, 2010 5:09 am


Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 1 guest

cron