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Need help with translation of DNA

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Need help with translation of DNA

Postby customx » Thu Jul 29, 2010 10:26 pm

I had a test today but was a bit confused on how to translate the mRNA into a protein. I understand how the whole process works, but I think my teacher threw us a curve. The mRNA strand was AUG AAG AUG .... UGA UGU CGA UGA

The ... represents a bunch of mRNA that coded to amino acids, but my question revolves around the two ends. If there are two methionine (MET), are they both part of the protein? Does one of them get cut out? The same goes for the two stop codons. This is my first post here, so thanks to anyone who helps out :D
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Postby JackBean » Fri Jul 30, 2010 6:24 am

the START codon is simply codon for Met, so if is it in the middle of the gene, it is just Met. Usually the first START codon is true START codon (but codin for Met anyway) and unless he didn't provide data about posttranslational modifications, you should leave it there.
The same with STOP codon. The first works as STOP codon and the others are just for case... Because you have already terminated the translation, you have no reason to use the other STOP codons ;) Anyway, if there was some mutation, changing the STOP codon into something else, just the next STOP codon would take the work.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re: Need help with translation of DNA

Postby customx » Fri Jul 30, 2010 3:58 pm

I included both Met, but didn't include any more amino acids after I saw the first Stop codon. In the future, once I encounter the first Stop codon, everything else after that isn't translated, correct? Oh, and the Stop codon is never translated into a protein either, is it? Thanks!
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Postby JackBean » Sat Jul 31, 2010 8:28 am

no, STOP codn is not translated, there is terminating factor instead
http://www.biolib.cz/en/main/

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