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How to use Bioedit?

Everything on bioinformatics, the science of information technology as applied to biological research.

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How to use Bioedit?

Postby nettle » Sun Jul 18, 2010 7:03 am

Hello,

I usaully use Bioedit for editing DNA sequences to use in my project of phylogenetic analysis. This time I had a problem and can't solve it.
Its just that I got a gene sequenced using three primers, two were forward primers and third one is reverse. Now, I dont understand how to combine all three obtained sequences of the target gene using Bioedit?

Please Help.
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Postby JackBean » Sun Jul 18, 2010 5:47 pm

well, the one you do reverse complement (I think Shift+Ctrl+Space) and then you can either use ClustalW under Accesory Applications, but that usually tries to put all the sequences together at one place, so you will have to do pairwaise alignments and put the sequences together ;)

Anyway, you may try to look here
http://www.mbio.ncsu.edu/BioEdit/bioedit.html
in the middle of page is BioLign, which might be useful for you, but I have not tried it yet ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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