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Gluten-free and Vaccines

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Gluten-free and Vaccines

Postby tmerezko » Mon Jun 21, 2010 5:10 pm

Just wondering if anyone out there has found a connection between the dTaP vaccine and developing celiac disease from it?
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Postby canalon » Mon Jun 21, 2010 9:26 pm

That's a new one.
Never heard of it and I smell a MMR/autism kind of controversy. Care to point toward some place where this is suggested. Peer reviewed literature preferred.
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Re: Gluten-free and Vaccines

Postby tmerezko » Tue Jun 22, 2010 6:08 pm

Canalon- Thanks for your reply.

I don't have much to go on as far as a connection. Here's some info. that I found on a website:
I know that internet searching can be dangerous and I am a big fan of peer reviewed. This article lists some references and I have not yet gone to the originals to read them. However, I was just posting this online to see if anyone had heard of a connection before.

I was interested because I have had problems/symptoms with my bowel's since giving birth 3 mo. ago. I had passed it off as a breastfeeding issue or a diet issue. However, after changing my diet the symptoms did not really improve. I was looking into Celiac's symptoms (which are the exact same as mine, although I have not been diagnosed yet), when I found this connection. I received the DTaP vaccine on the day I gave birth in the hospital. And after 2 days of gluten-free, my symptoms are virtually nill.

Thanks for any insight you have.
tmerezko - a 7th grade life science teacher

http://www.vaccinetruth.org/gluten.htm
What does the Acellular DTaP Vaccine have to do with Gluten?

Many Autistic children cannot digest the protein from gluten. It causes gastrointestinal, and
neurological symptoms.

There is an interesting connection between the gliadin peptide, (gluten) and pertactin. Pertactin is the protein used in the acellular DTaP vaccine. Following is an explanation of the connection, proceeding with various CDC information, and medical abstracts.

http://www.eurosur.org/celiacos/Shan.pdf

The primary sequence of the 33-mer gliadin peptide also had homologs among a few nongluten proteins. Among the strongest homologs were internal sequences from pertactin (a highly immunogenic protein from Bordetella pertussis) and a mammalian protein tyrosine phosphatase of unknown function. In both cases, available information suggested that this homology could have biological relevance. For example, the region of pertactin that is homologous to the 33-mer gliadin peptide is known to be part of the immuno-dominant segment of the protein (34). In the case of the phosphatase homolog, the protein is known to undergo vesicular trafficking into
the cytoplasmic Golgi (35). By analogy with the current understanding of how gliadin peptides
are acquired by HLA-DQ2 via a tTGase-mediated pathway (3), we hypothesized that these Pro-Gln-rich segments of both pertactin and the phosphatase are likely to be high-affinity tTGase substrates.
To test this hypothesis, we synthesized the corresponding peptides and measured the selectivity
of tTGase for these. As predicted, both peptides were found to be good substrates of
tTGase (36). Studies by Pastan and co-workers showed that tTGase plays a key role in
receptor-mediated endocytosis of several biologically important proteins (37). Therefore,
we therefore propose that the biological activities of both pertactin and the phosphatase

depend on tTGase-mediated trafficking. Analogous to gliadin in Celiac Sprue, pertactin
elicits a vigorous antibody response because it reacts with tTGase on the extracellular surface of antigen presenting cells to produce a long-lived intermediate that is internalized via endocytosis and presented to the immune system via the class II MHC-mediated pathway. If this analogy holds, then, drawing upon the Celiac Sprue analogy, one could predict that tTGase-mediated endocytosis might be a highly effective mechanism for oral vaccination with the use of immunogenic peptide epitopes, as long as they are intrinsically resistant to the action of
pepsin, trypsin, chymotrypsin, and elastase.

By flanking such epitopes with proteolytically stable high-affinity tTGase substrates [e.g., the sequence PQPQLPYPQPQLP from gliadin (21)], they could be protected from exposure to potent pancreatic and intestinal exopeptidases and would therefore have sufficiently long half-lives to permit efficient stimulation of the gut-associated lymphoid system. Secondary structural studies using circular dichroism spectroscopy on the 33-mer gliadin peptide and its homologs from pertactin and the tyrosine phosphatase reveal that these peptides have strong type II

polyproline helical character (fig. S2).
In addition to reinforcing the proteolytic resistance of these peptides, the type II polyproline helical conformation is typical of peptides bound to class II MHC proteins and is likely to enhance their binding to these proteins (38, 39). The abundance and location of proline
residues is a crucial factor contributing to the resistance of the 33-mer gliadin peptide to
gastrointestinal breakdown. Therefore, we hypothesized that a prolyl endopeptidase could catalyze breakdown of this peptide, thereby diminishing its toxic effects. Preliminary in vitro studies with short gliadin peptides and the prolyl endopeptidase (PEP) from Flavobacterium meningosepticum supported this hypothesis (21).
The ability of this PEP to cleave the 33-mer gliadin peptide was evaluated in vitro (Fig. 4A) and in vivo using the rat intestinal perfusion model (Fig. 4B). In the latter assay, the synergistic effect of PEP and BBM peptidases was evident. Moreover, the T cell stimulatory potential of PEP treated peptide was shown to decrease rapidly (Fig. 4C). Given the preference of PEP for
Pro-Xaa-Pro tripeptides (40) and the abundance of this motif in immunogenic peptides from gluten (41), these results highlight the potential of detoxifying gluten in Celiac Sprue patients by peptidase therapy.


References and Notes
1. D. Schuppan, Gastroenterology 119, 234 (2000).
2. S. N. McAdam, L. M. Sollid, Gut 47, 743 (2000).
3. L. M. Sollid, Annu. Rev. Immunol. 18, 53 (2000).
4. M. Maki, P. Collin, Lancet 349, 1755 (1997).
5. G. Corrao et al., Lancet 358, 356 (2001).
6. The family of storage proteins in cereals also includes secalins from rye and hordeins from barley. For review see [H. Wieser, Bailliere’s Clin. Gastroenterol. 9, 191 (1995)].
7. W. K. Dicke, N. A. Weijers, J. H. van der Kramer, Acta Paediatr. 42, 34 (1953).
8. H. Arentz-Hansen et al., J. Exp. Med. 191, 603 (2000).
9. R. P. Anderson, P. Degano, A. J. Godkin, D. P. Jewell, A.V. Hill, Nature Med. 6, 337(2000).
10. W. Dieterich et al., Nature Med. 3, 797 (1997).
11. The extracellular tissue transglutaminase is the primary autoantigen in Celiac Sprue (10).
12. L. M. Sollid et al., J. Exp. Med. 169, 345 (1989).

13. The class II HLA DQ2 molecule is expressed in 90% of Celiac Sprue patients (12).
14. E. H. Arentz-Hansen, S. N. McAdam, . Molberg, C. Kristiansen, L.M. Sollid, Gut 46, 46 (2000).
15. Materials and methods are available as supporting online material on Science Online.
16. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
17. H. Arentz-Hansen et al., Gastroenterology 123, 803 (2002).
18. L. Shan et al., unpublished data.
19. G. M. Gray. Handbook of Physiology, R.A. Frizzell, M. Field, Eds. (Oxford Univ. Press, New York, 1991), pp. 411-420.
20. D. J. Ahnen, A. K. Mircheff, N. A. Santiago, C. Yoshioka, G. M. Gray, J. Biol. Chem. 258, 5960 (1983).
21. F. Hausch, L. Shan, N. A. Santiago, G. M. Gray, C. Khosla, Am. J. Physiol., in press.

22. K. W. Smithson, G. M. Gray, J. Clin. Invest. 60, 665 (1977).
23. Molberg et al., Eur. J. Immunol. 31, 1317(2001).
24. J. L. Piper, G. M. Gray, C. Khosla, Biochemistry 41, 386 (2002).
25. For example, the speci.city of tTGase for the -gliadin - derived peptide PQPQLPYPQPQLPY is .vefold higher than that for its target peptide sequence in
.brinogen, its natural substrate (24).
26. kcat/KM440 min 1mM 1 for LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF
compared with kcat/KM 82 min 1mM 1 for PQPQLPY (24) and kcat/KM 350
min 1mM 1 for PQPQLPYPQPQLPY.
27. G. D’Argenio, I. Sorrentini, C. Ciacci, G. Mazzacca,
Enzyme 39, 227(1988).
28. T cell clones and T cell lines were established from small intestinal biopsies of adult HLA DQ2-positive Celiac Sprue patients as described (8). Stimulation of T cells by peptides was measured by a [3H]-thymidine incorporation assay. HLA DQ2 homozygous Epstein Barr virus-transformed B cell lines were used as antigen presenting cells.

29. J. J. Boniface et al., Immunity 9, 459 (1998).
30. J. R. Cochran, T. O. Cameron, L. J. Stern. Immunity 12, 241 (2000).
31. K. Falk et al., J. Exp. Med. 191, 717 (2000).
32. M. Stienekemeier et al., Proc. Nat. Acad. Sci. U.S.A. 98, 13872 (2001).
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Postby canalon » Tue Jun 22, 2010 8:37 pm

First Vaccinetruth is not exactly a highly regarded organization among biologist and medical professionals, so I would be careful. And the link between gluten and autism is bunk, although some desperate parents of autistic children would like to believe. You can see more on the subject on this very good website: www.badscience.net

As for the link between gliadin and gluten it indeed seems legit, but you will notice simply that they prove that both can elicit similar reaction. NOT that gliadin will necessarily cause an immunological cross reaction between the 2 peptides. And there is a difference between the inability to digest some proteins and being injected with similar proteins in order to create an immune reaction. So this is far from proving that DTaP vaccination is presenting any risk.
However the benefit to you and your baby to be protected against pertussis, a very nasty disease that could kill or at least severely impact the health of you baby (it is nicknamed the 100 days fever, for its average duration), is strong and proven. And vaccination against pertussis has been switched from inactivated agent to acellular pertussis because it had significantly less side effects in this form. The vaccine is considered very safe and providing significant benefit to be given.

I cannot have any idea of what is your problem, and I suggest that you discuss that with a health professional (that do not include naturopath, homeopath or other "alternative medicine" practitioner) that can actually run some test and check if you really have what you believe you have, rather than a case of freaking from reading things on the internet. the later is highly contagious, hard to avoid, but can be in fact quite dangerous in some cases.
Patrick

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Re: Gluten-free and Vaccines

Postby tmerezko » Thu Jun 24, 2010 2:16 am

I know the link between gluten and autism is bunk as well as the link between vaccines and autism. I am aware of the faulty study.

My original inquiry was just to simply ask if anyone had heard about a connection between DTaP and gluten-sensitivity. I did not mean to act as an expert in the field.

Thank you for the time that you put into your reply. I hope that someone will test this one day in a study.
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Postby jsblue » Thu Jun 24, 2010 2:26 pm

Hi tmerezko,

A friend came across a similar issue and it turned out that she did have celiac disease. It's a genetic autoimmune disease that is usually triggered by a stress event, often times times that is surgery or a pregnancy. Improvement after going gluten-free seems to confirm this. However, you have to be consuming gluten in order for the tests to work if you really want to find out...

I am not a doctor but if going gluten-free helps my common sense tells me that you should stay gluten-free. Same is true for autism and gluten: friends of mine said it was all bogus but tried a gluten-free diet for their 5 year old son. The results were amazing. The most recent study by Dr. Susan Hyman only observed 14 kids and disregarded about half of them because they had a gluten intolerance. But ppl tend to read only head lines, and the headline said "no link between gluten and autism found".

Anyway, it all boils down to this: if going gluten-free helps you feel better I think it does not matter whether or not you have celiac disease. What counts is your well being. I am sure you are smart enough to make the right decision for yourself.
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Re: Gluten-free and Vaccines

Postby canalon » Thu Jun 24, 2010 10:53 pm

tmerezko wrote:I know the link between gluten and autism is bunk as well as the link between vaccines and autism. I am aware of the faulty study.

My original inquiry was just to simply ask if anyone had heard about a connection between DTaP and gluten-sensitivity. I did not mean to act as an expert in the field.

Thank you for the time that you put into your reply. I hope that someone will test this one day in a study.


The sides effect as listed by the CDC are listed here and there is no mention of gluten sensitivity among those. As I said above this does not mean that there can be no link, but it must not have been reported. One thing that you can do is to report the problem, most countries do have a reporting schemes for adverse side effects after vaccinations and use of other medications.
Even better, have your family doctor/GP do that for you and start investigating your problem. This will help insure that the reporting is complete and that whatever is found might be added. If there is a link, that might help others. However, keep in mind that correlation is very different from causation, and that what you are now going through might be completely unrelated to your vaccination, except temporally.
Patrick

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Postby Darby » Sun Jun 27, 2010 2:11 pm

There are some studies which suggest that the genes / alleles associated with autism might code for genes also active in digestion, so a problematic code might produce both brain and digestive issues.

How that would relate to the vaccine, no idea.
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