Login

Join for Free!
111304 members


Limiting factor on organism density in submerged culture?

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderator: BioTeam

Limiting factor on organism density in submerged culture?

Postby Tsathoggua » Sat Jun 19, 2010 5:39 pm

Greetings people, first post here.

In the case of submerged cultures, in this case it is concerning organisms immobilized in Ca alginate microspheres, what is the limiting factor, where density of organism in the medium is concerned?

In this case the organism is a fungus with a high oxygen demand, so that is one factor, but O2 is easily suppliable, and fluoroalkanes or fluorinated ethers/aromatics can fulfill a role as oxygen carriers due to the high soluability of gaseous O2 in such solvents.

What I want to know though, is with a given quantity of immobilized mycelia in the liquid medium, is there any good reason it cannot be increased, and the concentraton of nutrients increased also.

What is the limit here? doubled? tripled? or can one go on increasing the concentration until viscosity starts buggering up oxygen transport? the more organism that can be packed into one bioreactor, the better.
Tsathoggua
Garter
Garter
 
Posts: 2
Joined: Sat Jun 19, 2010 5:26 pm

Postby JackBean » Sat Jun 19, 2010 5:54 pm

each compound can be poisonous, just the level decides
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5624
Joined: Mon Sep 14, 2009 7:12 pm

Postby Tsathoggua » Sat Jun 19, 2010 6:01 pm

There are going to be limits possibly as to citrate in the medium specifically, although it isn't essential, as citrate acts as a chelator and destabilizes the alginate beads.
Tsathoggua
Garter
Garter
 
Posts: 2
Joined: Sat Jun 19, 2010 5:26 pm


Return to Microbiology

Who is online

Users browsing this forum: No registered users and 4 guests

cron