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Lysozyme liquid preparation

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Lysozyme liquid preparation

Postby magicsiew » Wed May 12, 2010 2:01 am

Hi, anyone know how to prepare lysozyme liquid from the crystalline powder? The powder is bought from Sigma.
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Postby magicsiew » Wed May 12, 2010 2:05 am

I will need to prepare 50mg/ml
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Postby JackBean » Thu May 13, 2010 9:51 am

I would weight 50 ml and dissolve in 1 ml of water or buffer, if you want ;) :lol:
http://www.biolib.cz/en/main/

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Postby magicsiew » Fri May 14, 2010 4:20 am

Yes, that is the basic calculation. However, how about the amount of TRIS-HCL required to be added? The handbook only say using 10mM of TRIS-HCL, without stating the volume to be added. What I have done is I just use the autoclaved ultra clean water without adding the TRIS-HCL. The result coming out later was not so good.
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Postby JackBean » Fri May 14, 2010 6:59 am

just prepare the buffer and use it then to dissolve the lysozyme ;)
http://www.biolib.cz/en/main/

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