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Determining an Ig percentage from crude serum w/ELISA

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Determining an Ig percentage from crude serum w/ELISA

Postby bemardus » Tue May 04, 2010 9:17 pm

Hey,

So I'm curious how to figure out the percentage of total protein in crude serum that is attributed to a specific Ig. All I know is that it has to do with an ELISA. Is BSA involved? Some sort of working reagent? Any clue here guys?
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Postby JackBean » Thu May 06, 2010 7:42 am

maybe sandwich ELISA?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re: Determining an Ig percentage from crude serum w/ELISA

Postby researcher4life » Fri May 07, 2010 6:42 pm

We have two ELISAs for IgE. One is a Mouse total IgE and the other is OVA-specific IgE ELISA.

Principle of each assay
The mouse IgE uses a mouse monoclonal antibody to IgE attached to the wells as a capture antibody and then HRP conjugated IgE in the presence of substrate is used as a detection antibody.

The OVA-IgE uses a monoclonal antibody to mouse IgE heavy chain attached to the wells as a capture antibody and then biotinylated ovalbumin and streptavidin-peroxidase in the presence of substrate is used as a detection antibody.

If you go to each on our website you can view the product insert and learn more about assembling an ELISA for your application.

*I'm affiliated with MD Bioproducts
Last edited by JackBean on Fri May 07, 2010 6:48 pm, edited 1 time in total.
Reason: spam removed
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