Determining an Ig percentage from crude serum w/ELISA

[bemardus]

Hey,

So I'm curious how to figure out the percentage of total protein in crude serum that is attributed to a specific Ig. All I know is that it has to do with an ELISA. Is BSA involved? Some sort of working reagent? Any clue here guys?

[JackBean]

maybe sandwich ELISA?

[researcher4life]

We have two ELISAs for IgE. One is a Mouse total IgE and the other is OVA-specific IgE ELISA.

Principle of each assay
The mouse IgE uses a mouse monoclonal antibody to IgE attached to the wells as a capture antibody and then HRP conjugated IgE in the presence of substrate is used as a detection antibody.

The OVA-IgE uses a monoclonal antibody to mouse IgE heavy chain attached to the wells as a capture antibody and then biotinylated ovalbumin and streptavidin-peroxidase in the presence of substrate is used as a detection antibody.

If you go to each on our website you can view the product insert and learn more about assembling an ELISA for your application.

*I'm affiliated with MD Bioproducts