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Slide problem

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Slide problem

Postby Orome » Fri Apr 23, 2010 8:37 am

Hey guys,
It’s my first thread in this great forum and I hope you can help me out of a fix. A part of my master thesis consists of doing immunohistological staining of “whole-retina-flatmounts”. In this experiment I dissect the retina from a mouse eye, make some cuts to bring the cup-shaped structure into a flat shape and do some staining with it. Everything happens on a microscope slide.
To create a little “pit” where I can put my retina flatmount in take a slide and glue two coverglasses on top of it. The area between the two slides forms a depression with flat ground. Which can be covered with a third coverglass. The problem with this technique is that it sometimes happens that the retina is sucked by capillary action under one of the glued coverglasses. If this happens I can throw my preparation away.
My question is if anybody knows a distributer of special microscope slides containing such a “ flat pit”. The usual “depression slides” (like this one: http://www.mccronemicroscopes.com/store ... gory_id=74) are not beneficial for me cause the pit has a spherical shape but the retina has to be completely flat. If these special slides does not exist, maybe somebody has a different idea for my problem.
Thank you very much

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Re: Slide problem

Postby jonmoulton » Fri Apr 23, 2010 5:48 pm

As I recall a hemocytometer has a flat surface and a raised edge which can hold liquid and be covered by a cover slip. I don't know if the space between the cover slip and the grid would be thick enough for your retina sample. This might be worth considering.

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