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Type ii restriction endonucleases

Everything on bioinformatics, the science of information technology as applied to biological research.

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Type ii restriction endonucleases

Postby Soni » Tue Apr 20, 2010 10:30 pm

Does anybody know how three different type ii restriction endonucleases can cut at the same sequence but at different points to yield three different set of products?
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Postby JackBean » Wed Apr 21, 2010 9:22 am

That's pretty simple. The ER can produce either blunt ends or sticky ends with either 5'- or 3'-overhang. Thus if you had sequence GAATTC you can have:

G...AATTC
CTTAA...G

GAA...TTC
CTT...AAG

GAATT...C
C...TTAAG
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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