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Restriction Mapping for Plasmids

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Restriction Mapping for Plasmids

Postby Molekul » Tue Apr 20, 2010 8:49 pm

I'm understanding the concept, the process, and the purpose. I'm not able to draw it out.
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Code: Select all
Enzyme Digest Length of Fragments (Kilobase)
EcoRI 3.71, 4.11
NcoI 7.82
EcoRI & NcoI 3.71, 2.15, 1.96


How do I do the circular mapping? Where do I start with the data.
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Postby JackBean » Tue Apr 20, 2010 10:01 pm

well, apparently the plasmid has 7.82 kbps and is cut once by NcoI and EcoRI. Apparently, the NcoI cuts in the fragment 4.11 of EcoRI digest. I think, that's it :)
http://www.biolib.cz/en/main/

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Postby Molekul » Tue Apr 20, 2010 10:03 pm

:) Thanks, I understand that, but are there specific steps to drawing the circle itself. Can this circle be drawn multiple ways and still be correct?
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Postby Molekul » Wed Apr 21, 2010 12:47 am

Anyone? I've been mapping the restrictions in the circular format, but I'm just not sure if it's correct.
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Postby Molekul » Wed Apr 21, 2010 3:15 am

If people want I can post drawings, I read the rules, I'm not asking for direct answers, just steps to completion.
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Postby JackBean » Wed Apr 21, 2010 9:34 am

Maybe there are not people all the time. We have to sleep ever too ;)

I'm not sure, what's the problem. Just make a circle, put on two EcoRI and one Nco restriction places in correct orientation ;)
http://www.biolib.cz/en/main/

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Postby Molekul » Wed Apr 21, 2010 6:21 pm

Why two EcoRI and one NcoI?
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Postby JackBean » Wed Apr 21, 2010 6:32 pm

because you have two and one piecies after digestio by EcoRI and NcoI, respectively.
http://www.biolib.cz/en/main/

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Postby Molekul » Wed Apr 21, 2010 6:59 pm

Ok I think I got it. Thank you sir. I don't see a rep button, but I give you props. Much appreciated it.
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