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DNA sequencing problem that I'm absolutely stumped by

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DNA sequencing problem that I'm absolutely stumped by

Postby crouch88 » Tue Apr 20, 2010 2:18 am

Hey guys. It would be awesome if you could help me with this question. I have absolutely no clue how to go about it. :(

Consider the following DNA sequence:
AATATCAATGAATGTCATAGTGTCACTAAGGATGC

Assume you have available the follwing resources to hand-annotate the sequence above:

EST E1 (expressed sequence tag)
: CAATGAATTG
EST E2: CCTTAGT
Proteins: Met Asn Cys His

Identify:
5' UTR
3' UTR

And the full length mRNA.
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Postby JackBean » Tue Apr 20, 2010 6:55 am

I will split you sequence a little

you have:
AATAT
than there is EST1 (although with one mutation, you probably wrote it wrong ;)
CAATGAATGT
than some other sequence
CATAGTGTC
and EST2 (in reverse complement;)
ACTAAGG
and the end...
ATGC

but I'm littlw confused with the ORF, it doesn't seem to work. Just look for all ATGs and subsequent stop codons
http://plato.stanford.edu/entries/infor ... icCode.png

(look if you have correct sequence;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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