Unknown Id. Bacteria

[nikolette21]

Recently started doing unknown identification tests in lab. So far I have found out it is gram neg. Bacilli & a gram pos. cocci. My one clue is "patient presented with abdominal pain & tenderness" & the blumberg sign. Preliminary diagnosis is peritonitis. I was thinking it might be escherichia coli or klebsiella pneumonia and the gram pos. could be staph. aureus? But I'm not sure and I dont know what my next step should be.

[JackBean]

the best next step is PCR, worst are some biochemical tests

[bebe2]

hoping to get alitle help with an unknow bacteria that i have. i have done several different tests and i have narrowed it down to either Bacillus cereus or Bacillus megaterium. wondering if it could possibly be Bacillus subtilis. but i think i have cancelled that one out. here is my test and results see what you might think.

glucose acid: +
glucose gas: -
lactose acid: -
lactose gas: -
mannitol acid: -
mannitol gas: -
sucrose acid: +
sucrose gas: -
methyl red test: +
voges-proskauer test: +
catalase: +
oxidase: +
hydrogen sulide production: -
citrate: -
phenylalanine deamination: -
starch: +
gelatine: +
indole: -
urea: +

leaning more towards cereus.

thanks
bebe

[Jillo725]

@nikolette21 - i would say E.coli for your G-. if you have staph aureus try a beta-hemolytic test. I forgot what they're called. to distinguish between s.aureus and s. epidermis.

@bebe2 - when you did a gram stain, were the bacteria in chains or not? that would distinguish them from each other.