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how to SAVE in fasta?

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how to SAVE in fasta?

Postby turlaa » Thu Apr 15, 2010 8:30 pm

Hi guys!
I have probably silly problem. I work on Join Genome institute software and I have my results in fasta format (i mean it looks like fasta) BUT I don't know how to save it in fasta format so I can open it in mega-they just don't have saving option. Any ideas?
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Postby JackBean » Thu Apr 15, 2010 8:41 pm

What doesn't have saving option?
http://www.biolib.cz/en/main/

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Postby turlaa » Thu Apr 15, 2010 8:44 pm

well joint genome institute web page they show me my gene list and I have option to see it in fasta format but I can't save it I just have empty page with only fasta on it.
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Postby JackBean » Thu Apr 15, 2010 8:45 pm

than just copy and paste into text file :lol:
http://www.biolib.cz/en/main/

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Postby turlaa » Thu Apr 15, 2010 8:50 pm

well it isn't that easy cause I have to open it later in mega4 so it have to be blahblahblah.fasta not blahblahblah.txt and here is my problem cause all convertors just show output but don't save with the right extensions
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Postby turlaa » Thu Apr 15, 2010 8:51 pm

thank you for bearing with me!
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Postby JackBean » Thu Apr 15, 2010 9:01 pm

My mentor told me in the beginning: "Just forget about what is Microsoft pushing you into, forget everything about the extensions."
This is just plain text, you can give it any extension you want - *.txt, *.fasta, *.fsa, *.fas, *.tab... whatever. I have one file ending with .friends (by the Tv series;) :lol: But you have it to be just plain text in format:

>your_ID whatever you want to have here
SEQUENCEOFYOURPROTEINMOSTLIKELYWITH60
LETTERSPERLINE(ORMOREORLESS),BUTDEFINIT
ELLYWITHOUTSPACES
>ID2 description of your second sequence
ANDHERESEQUENCEOFYOURSECONDPROTEINA
GAINWITHTHESAMEFORMATING

OK? So, just copy your sequences,put it into Notepad (not Word!!!) and put Save As and choose "All files" and into the name put "something.fasta" or .fsa or .fas, that doesn't matter so much, the format is important ;)
http://www.biolib.cz/en/main/

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Postby turlaa » Thu Apr 15, 2010 9:06 pm

I will try just now! thank you!
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Postby JackBean » Thu Apr 15, 2010 9:16 pm

You're welcome :) One doesn't understand, how much important the formatting is, unless s/he doesn't write some program...
http://www.biolib.cz/en/main/

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Postby turlaa » Thu Apr 15, 2010 9:46 pm

Oh I am far from that! I'm very thankful for your help. anyway MEGA decided not to work with me good format or wrong(it just show some error)
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Postby fcs » Wed Aug 11, 2010 4:09 am

What kind of error did you see from MEGA?
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