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concentration of isopropanol?

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concentration of isopropanol?

Postby sixpence » Sun Apr 11, 2010 7:33 pm

Why is the concentration of isopropanol never listed for any protocols? Is it just assumed to be 100%? I'm trying to do isopropanol precipitation.
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Postby JackBean » Sun Apr 11, 2010 7:40 pm

yeah, unless otherwise stated, it's probably 100%
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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Re: concentration of isopropanol?

Postby sixpence » Sun Apr 11, 2010 7:42 pm

so no diluting from the stock? I'm still trying to find the stock.
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Postby JackBean » Sun Apr 11, 2010 8:27 pm

You're salting out the proteins? I think not, because then you had to add larger volume and thus more dilute your sample. What is your protocol?
http://www.biolib.cz/en/main/

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Postby pavi » Sat Apr 24, 2010 12:35 pm

WAT R U PRECIPITATING OUT.... NUCLEIC ACID OR PROTEIN...
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Re: concentration of isopropanol?

Postby Kristenmickel » Fri Apr 30, 2010 7:45 am

I don't know much about this subject but wish to know more..
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