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DNA coding strand

Genetics as it applies to evolution, molecular biology, and medical aspects.

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DNA coding strand

Postby bomb4268 » Thu Mar 11, 2010 3:15 pm

This is a coding strand of DNA Write in the bases necessary to create the template strand. Find the -35 concensus region. Find the -10 TA rich region show where transcription begins and write the mRNA transcript. Find the Shine Dalgarno sequence Identify the stop codon Identify the ATG start and translate the peptide. Add an insertion of one A immediately following the ATG start and then retranslate the protein. What happens? 5’AACCTTGTTGACATGGACCATGCAATCCTTAGTATAATGCAATAGCACATCCTAGGAGGTTTGATCCTATGATGATGATGATGATGATGTGATAATCCACAGCCGCCAGTTCCGCTGGCGGCATTTTTTTTTTTTTTTTTATGCAGTCGTCAGTTCA3’
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Re: DNA coding strand

Postby mamoru » Thu Mar 11, 2010 4:16 pm

bomb4268 wrote:What happens?

When the mutation of this gene is expressed, is causes the bearer to open his biology text book to the page with the genetic code table and do his own homework, or at least attempt to and post the results and ask specific questions about the parts he doesn't understand. It's a pretty useful mutation.
"Empathise with stupidity, and you're halfway to thinking like an idiot." - Iain M. Banks
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Postby JackBean » Fri Mar 12, 2010 7:50 am

there are plenty of web pages and softwares to do such things. Just look for some...

(or at least write your sequence correctly!!! :twisted:)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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