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In situ RNA-RNA probes

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In situ RNA-RNA probes

Postby yungyungbee » Thu Mar 04, 2010 8:33 am

hi, i am new to use in situ hybridization

i made in situ RNA probes from PCR template and T7 RNA polymerase, the produced probes has 1.5ug/ul concentration. i loaded 3 ul of the sample to a normal TBE DNA gel, and the gel photo show a smear with 2 sharp band is the size of my template and the other band is the smear due to too much probes loaded??....or sth wrong in the process of producing the probe??

i would like to know whether i have succesffuly produce the probe or not....and should i dilute the probe before doing the in situ hybridization? and can it be simply diluted DEPC-treated water??.....

thanks a lot
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