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growth in dark

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growth in dark

Postby JackBean » Tue Feb 16, 2010 8:43 am

Let's grow some plant in dark, so it won't synthesize chlorophyll (why should it?) and doesn't photosynthesize. How can it then grow? Where does it take stuff for growth and everything?
I would be fine with e.g. bean, which has large seeds, but what about some oat?
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Postby jwalin » Tue Feb 16, 2010 11:41 am

i think for that you must refer to or look into the plants that grow in rainforest in darkness, help it will.
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Postby JackBean » Tue Feb 16, 2010 4:32 pm

I'm talking about complete darkness, not shadow
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Postby Darwin420 » Tue Feb 16, 2010 5:28 pm

Do it!!

It will grow to a certain extent by using the energy in the endosperm and then die once all the energy from the endosperm is used up. The seedling should be white in complete darkness theoretically, however, there may be very little amounts of chlorophyll expressed. But over all chlorophyll production is positively correlated with sun light.
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Postby JackBean » Tue Feb 16, 2010 5:58 pm

I did it already ;)

I have grown oat for two weeks. It was etiolated, but didn't seem, that it should die shortly...
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Re: growth in dark

Postby Darwin420 » Sat Feb 20, 2010 6:14 pm

Nice man, let me know when you start to notice cell death.
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Postby JackBean » Sat Feb 20, 2010 8:02 pm

I have already pure PAO from oat isolated, so I won't have many choices to see cell death :)
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Postby Darwin420 » Sun Feb 21, 2010 1:17 am

PAO?
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Postby mith » Sun Feb 21, 2010 4:55 am

You provided light when you peeked at it :D
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Postby JackBean » Sun Feb 21, 2010 4:19 pm

PAO = polyamine oxidase

mith: yeah :lol: but that was like 5 min per day ;)
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