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NADP+ and NAD+

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NADP+ and NAD+

Postby klou » Sat Feb 13, 2010 6:43 pm

I have no idea how to answer this question:

through genetic engineering, you have developed an unregulatable enzyme that can interchangeably use NAD+ or NADP+ in a redox reaction. What would be the physiological consequence(s) of having such an enzyme for an organism.

I though that it may have something to do with the regulation of the pentose phopshate pathway, but I'm really stuck.
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Postby JackBean » Sat Feb 13, 2010 7:17 pm

the ratio [NADP+]/[NADPH] in rat liver cells is about 0.014, whereas that of [NAD+]/[NADH] is about 700. That means, that you have favoured for oxidative break-down (NAD+) and reductive biosynthesis (NADPH).
If you had such enzyme, you would have mixed up the concentrations and all regulations diminished.

You're basically true about the pentose phosphate pathway

Cis or trans? That's what matters.
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