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NADP+ and NAD+

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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NADP+ and NAD+

Postby klou » Sat Feb 13, 2010 6:43 pm

I have no idea how to answer this question:

through genetic engineering, you have developed an unregulatable enzyme that can interchangeably use NAD+ or NADP+ in a redox reaction. What would be the physiological consequence(s) of having such an enzyme for an organism.

I though that it may have something to do with the regulation of the pentose phopshate pathway, but I'm really stuck.
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Postby JackBean » Sat Feb 13, 2010 7:17 pm

the ratio [NADP+]/[NADPH] in rat liver cells is about 0.014, whereas that of [NAD+]/[NADH] is about 700. That means, that you have favoured for oxidative break-down (NAD+) and reductive biosynthesis (NADPH).
If you had such enzyme, you would have mixed up the concentrations and all regulations diminished.

You're basically true about the pentose phosphate pathway
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
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