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My new experiment in DNA modification

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My new experiment in DNA modification

Postby hashemyemen » Sun Feb 07, 2010 1:30 pm

Hello, How Are You All??



Have you ever imagined, what will be the benefits of blocking the transfer of genetic information from the parents to the offsprings?

Have you ever thought, what will be the procedure to block the transfer of the genetic information from the parents to the offsprings?

Have ever dreamed this world without hereditary diseases?

Of course, the answer would be Yes, and would be No, but most probably, your answer will be No.


Now, being a biotechnologist, I'm pleased to give you short entry about my new research and idea about how to stop shifting the genetic information from the parents to the offsprings via nucleic acids.

Several hereditary diseases have not controled yet. Thus, the need to control them is quite important for the best life unoccupied with such diseases.

We know that DNA is the only one responsible for shifting these diseases from the parents to the offsprings via its processing in replication and transcription, thus the only one way to control these diseases is by controlling DNA's ability to do this transfer!

Moreover, DNA mutations can make the situation much more worst as they are responsible for many hereditary diseases.


In my experiment, I took a male mouse carrying black color, and jenny mouse carrying white color, after natural meeting, the first generation were carrying back and white color 50:50. The second generation were also having balck color, but 75:25 in compare to the white color.

This means, the black color is a genetic property that must be transferred from the parents to the offsprings, and this property must passed all the generations even to the last generation of the same family.
This is the way in which DNA use its ability to tansfer the hereditary diseases from generation to another generation.

In my last experiment, I took a male mouse carrying black color, and jenny mouse carrying white color, after natural meeting, and with some modification in their DNA and chromosomes in condensed from, the result was different! I repeated the same procedure and the result was also the same!
Unlike the previous one, this time all the mice got the white color!

This result lead me to the ability to stop shifting the genetic information and hereditary diseases from the parents to the offsprings.


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Postby JackBean » Sun Feb 07, 2010 1:40 pm

1) your pictures are a little cut off
2) did what? Did you publish that or is it just swagger?
http://www.biolib.cz/en/main/

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Re:

Postby hashemyemen » Sun Feb 07, 2010 1:48 pm

JackBean wrote:1) your pictures are a little cut off
2) did what? Did you publish that or is it just swagger?


No, That is already done, and my experiment but I'm still looking for the way to publish it.

And about the picture you can see it full from this link:-

http://img519.imageshack.us/img519/4293/snag0001.jpg

If any question, you can ask. But do not mention the word swagger for me, because I do not like that at all.
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Postby JackBean » Sun Feb 07, 2010 2:23 pm

OK, so how did you do it? Where do you work?
http://www.biolib.cz/en/main/

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Re:

Postby hashemyemen » Sun Feb 07, 2010 3:00 pm

JackBean wrote:OK, so how did you do it? Where do you work?



I did that in 4 steps:-

1- Isolation of the DNA.
2- Purification of the isolated DNA.
3- Modification of the purified DNA.
4- insertion of the modified DNA into a specialized host cell.

And I'm working in the Labs of the centre of Biotechnology and Microbiology

University Of Peshawar
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Postby JackBean » Sun Feb 07, 2010 3:14 pm

you did that in mouse, right? How did you specifically modify the DNA?
http://www.biolib.cz/en/main/

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Re:

Postby hashemyemen » Sun Feb 07, 2010 3:25 pm

JackBean wrote:you did that in mouse, right? How did you specifically modify the DNA?



Yes, Of course. I did that in a number of mice, not in a single mouse.

About how to modify DNA, that will take other several steps :-

Cutting using restriction enzymes ( EcoR1 ).
ligation using ligase enzymes.
Translocations and transpositions in the DNA sequences.
And the last, to store the DNA for 5 days before to insert it to the specialized host cell.

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Re: My new experiment in DNA modification

Postby sara135 » Tue Jul 12, 2011 1:50 pm

Okay, I'm having some problems with the Qiagen kit. Ive got frozen paraffin embedded tissues and modified the extracted dna. i ran two pcr amplifications at about 300bp and then 200bp. dna recovery is low after the bisulfite modification. My samples are showing nothing! Any ideas?
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