microRNA Isolation

[Labgrl]

Can anyone please tell me if microRNAs are isolated along with larger RNA molecules using the caesium chloride RNA extraction protocol?

I have previously extracted RNA from frozen cell line material using caesium chloride centrifugation. I am now interested in microRNA and was wondering if my previously extracted RNA would also contain these small molecules.

[jonmoulton]

Labgrl,

There is an active miRNA research subforum on BioForum. You might try your question there. I can't help with miRNA extraction steps, but they are often discussed in the siRNA, microRNA and RNAi subforum linked below.

http://www.protocol-online.org/forums/index.php?showforum=21

Regards,

- Jon

[microRNAmiRNA]

FOR miRNA microRNA ISOLATION SEE PUBLICATION

MicroRNA isolation and stability in stored RNA samples.
Mraz M, Malinova K, Mayer J, Pospisilova S.
Biochem Biophys Res Commun. 2009 Dec 4;390(1):1-4. Epub 2009 Sep 19. Review.

[JackBean]

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