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Looking for advice regarding decapod microsurgery

Debate and discussion of any biological questions not pertaining to a particular topic.

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Looking for advice regarding decapod microsurgery

Postby Tex » Fri Jan 22, 2010 4:05 am

I'm doing microsurgery on young, live male decapods (.3g-.5g) and am having trouble finding forceps that will grip and extract the vas deferens through muscle tissue without slipping. The forceps I am now using have a straight tip (.05mm x .2mm) and are the perfect size. While I am comfortable working with a dissection microscope, getting used to forceps this small has been an obstacle. Even with a light touch the vas deferens slips through the tips, and the slightest bump against the carapace bends the tips. It's like trying to pull a thread of oil out of a block of ballistics gelatin. Is there a specific type of forceps I should be using for this kind of work, and if so what? I'm sure a lot of this boils down to a learning curve for technique, so any help on where to find additional resources for this type of work is also greatly appreciated, as would good reference sources for microsurgical techiques.

Tex
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