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chimeric PCR. How to avoid it?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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chimeric PCR. How to avoid it?

Postby inaki » Mon Jul 18, 2005 9:08 am

Hi,

I'd like to ask some advice on how to avoid chimeric PCR artifacts. I'm amplifying using primers from 2 different DNA fragments that have high homology and I sometimes have the PCR jumping across and giving me a chimeric artifact

Thanks
inaki
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