Biology-Online • View topic - Quantifying DNA Damage via Radiation.

Join for Free!
122491 members

Quantifying DNA Damage via Radiation.

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderator: BioTeam

Quantifying DNA Damage via Radiation.

Postby ishy » Thu Nov 26, 2009 6:19 pm

I'm trying to compare a couple studies that quantify DNA damage via radiation, but unfortunately, the key studies measure in very different ways. One article discusses SSB/bp/Gy (single stranded breaks per base pair per gray), and a couple others discuss yeilds of SSB in umol/J (micromoles per Joule).

I've made an attempt to find a way to convert between the two methods so I can actually compare the values, but I'm stumped. The following is that attempt:

In the first case, we have a yeild of Single Stranded breaks in micromoles per Joule:


To rephrase this in terms of SSBs, apply Avogadro Constant tremembering umol, not mol:

6.022x10^17 SSB/J

And that's as far as I get on this side of the problem. But there's another side of the problem (oh joy):


Now, this phrasing is just unpleasant as we are dealing with single stranded breaks in a single stranded scenario. So if there's 1 SSB/bp, there's 1 SSB/2nuc (nucleotides).


And 1 Gy = 1 J/kg. So it follows that:



SSB/2nuc * kg/J

and since we want this to approach what we've got up top, I'm going to collect the SSB and J to rewrite:

SSB/J * kg/2nuc

soooooo, we're left trying to equate, or find a conversion factor for:




Is this possible?
Posts: 1
Joined: Thu Nov 26, 2009 6:07 pm

Return to Molecular Biology

Who is online

Users browsing this forum: No registered users and 0 guests