Login

Join for Free!
119317 members


Cloning: Preserving DNA of a dead eagle

Genetics as it applies to evolution, molecular biology, and medical aspects.

Moderator: BioTeam

Cloning: Preserving DNA of a dead eagle

Postby rturu » Mon Nov 23, 2009 7:35 pm

Would like to preserve the DNA for the long term for perhaps future cloning.
I read on the news a 100 year old or something Tasmanian tiger that was preserved simply in ethanol, still has viable DNA in it.

Which would be better for preserving DNA:

A jar of ethanol + dry ice (to create a very cold liquid) (would the dry ice remain solid in the ethanol or would it be prone to disperse into the ethanol and dilute it due to room temperature or a hot day?)

OR

A jar of 98% ethanol (better bacteria destroying properties?)

OR

Something else

P.S: I do not want the high upkeep and management of liquid nitrogen (it loses about half a liter a day?), so these are the other options I am looking at Thanks!
rturu
Garter
Garter
 
Posts: 11
Joined: Mon Aug 17, 2009 8:09 pm

Postby JackBean » Tue Nov 24, 2009 4:37 am

If will you keep it at room temperature, than you will lose even the solid CO2, so the question is, whether you wish to cool it or keep it at RT. I think formaldehyde solution is usually used for animal preservation, isn't it? Althrought that will probably affect the DNA...
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5692
Joined: Mon Sep 14, 2009 7:12 pm

Postby mith » Wed Nov 25, 2009 8:10 pm

Sequence the genome, make redundant copies digitally. Or you can just freeze it.
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr
User avatar
mith
Inland Taipan
Inland Taipan
 
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN



Return to Genetics

Who is online

Users browsing this forum: No registered users and 1 guest