Login

Join for Free!
119261 members


Unknown bacteria

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderator: BioTeam

Unknown bacteria

Postby Hmicro » Mon Nov 23, 2009 12:39 am

I am very confused and becoming upset. I NEED HELP
These are my test results can someone give me an idea of what it could be?
Gram +
Cocci
Sucrose +
Glucose +
Lactose +
Mannitol +
Mehtyl Red +
VP test +
Catalase -
Starch -
casein -
fat hydrolysis +
urea -
kligers iron -
citrate-
phenylalanine -
litmus milk- acid reaction
What can this be please please help
Hmicro
Garter
Garter
 
Posts: 8
Joined: Mon Nov 23, 2009 12:29 am

Re: Unknown bacteria

Postby azarulez » Mon Nov 23, 2009 2:57 am

why don't you give a try using API kit? it's more easier to identify unknown bacteria..
azarulez
Garter
Garter
 
Posts: 10
Joined: Sat Apr 11, 2009 4:01 pm

Postby Hmicro » Mon Nov 23, 2009 3:07 am

What is a API kit?
Hmicro
Garter
Garter
 
Posts: 8
Joined: Mon Nov 23, 2009 12:29 am


Re:

Postby azarulez » Mon Nov 23, 2009 3:24 am

Hmicro wrote:What is a API kit?


API strips give accurate identifications based on extensive databases and are standardized, easy-to-use test systems. The kits include strips that contain up to 20 miniature biochemical tests. All are quick, safe and easy to perform.

link: http://www.biomerieux-usa.com/servlet/s ... _PRD_USA_5
azarulez
Garter
Garter
 
Posts: 10
Joined: Sat Apr 11, 2009 4:01 pm

enteroccus facalis

Postby Hmicro » Mon Nov 23, 2009 11:14 pm

Okay i think i found my unknown bacteria. Now i have to write a conclusion based on my test. I don't know how to begin this. Can someone help me . Based on my test results i think i can confirm its Enterocccus F. HELP this report is due in a 2 days :(
Hmicro
Garter
Garter
 
Posts: 8
Joined: Mon Nov 23, 2009 12:29 am

Enteroccoc F

Postby Hmicro » Mon Nov 23, 2009 11:41 pm

Does it ferment Glucose what happens with methyl red and VP Test. Does hydrolysis starch, casein, fat, urea, Klingers iron?
Hmicro
Garter
Garter
 
Posts: 8
Joined: Mon Nov 23, 2009 12:29 am

Postby MrMistery » Thu Nov 26, 2009 6:21 pm

Please post all your questions regarding the same thing into the same thread, do not start a new topic every time.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
User avatar
MrMistery
Inland Taipan
Inland Taipan
 
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)

Re: Unknown bacteria

Postby Hmicro » Sun Nov 29, 2009 9:49 pm

It ferments glucose, +for VP + for methyl red test, does not hydrolysis starch, casein yes, fat yes and urea no, klingers iron no
Hmicro
Garter
Garter
 
Posts: 8
Joined: Mon Nov 23, 2009 12:29 am

Postby Hmicro » Mon Nov 30, 2009 2:15 am

What happens with Enteroc. Fac. with
MP
VP
Casein
Fat
Urea
Kliglers Iron
Citrate
Phelalkine
Hmicro
Garter
Garter
 
Posts: 8
Joined: Mon Nov 23, 2009 12:29 am

Postby JackBean » Mon Nov 30, 2009 2:44 am

what about some rRNA or tRNA or anything sequencing? It's faster and more accurate ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5692
Joined: Mon Sep 14, 2009 7:12 pm

Postby Hmicro » Mon Nov 30, 2009 3:12 am

That is not part of our lab testing. I think my unknown is E. faecalis. I just need help finding out if it is postive for methyl red and VP also if it hydrolysis exoenzymes like statch, casein, fat, tryptophan,urea, kiligers iron, citrate and phenalalkine
Hmicro
Garter
Garter
 
Posts: 8
Joined: Mon Nov 23, 2009 12:29 am

Re: Unknown bacteria

Postby billw » Tue Dec 01, 2009 3:30 am

I'm in the process of writing a bacteria matching program, which you might find helpful. (And it would also help me out to have someone give it a try.)

http://moclips.net/microti/microti.php

It only has about 50 bacteria in the database at the moment, but it does give E. faecalis as the best match based on the info you have given. You can drill down to comparisons between the information you enter and the bacteria that appear to be the closest matches (which is mostly info from Bergeys, but is at least all in one place).
http://microbiology101.com/
billw
Garter
Garter
 
Posts: 14
Joined: Tue Dec 01, 2009 3:23 am

Next

Return to Microbiology

Who is online

Users browsing this forum: No registered users and 1 guest