help me..how to cultivate bacteria from lyophilized cell

[azarulez]

If you can, try to not use all your lyophilised sample at once. In case it would not work.

so, how much I need to use, I mean how many gram or miligram to resuspend it with water?

[canalon]

Depends how much you have.As for the amount of resuspended solution, the answer is enough. As in enough to plate it. This is definitely not exact science. What you want is to have a cell suspension in a liquid medium, the rest you can only guess.