Debate and discussion of any biological questions not pertaining to a particular topic.
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I'm doing a biology related science fair project, and needed some help to find some information.
This is the Project
To genetically splice the breast cancer cells and destroy or nullify the duplication gene. This will be done via gene splicing using restricting enzymes that attack duplicating genes. The desired result would be to see a decline in the rate of duplication.
I) 2 batches of breast cancer cells will be made.
II) One batch will recieve the treatment and the other will be allowed to develop naturally.
III) Then the 2 batches will be compared via microscope and estimation.
I hope to see a difference in the 2 batches.
So that is the rough overview of my project, But it raises a few questions, and i hope that you all can help in pointing me in the right direction.
How do we identify breast cancer cells from regular cells ?
Which is the duplication gene ?
What restriction enzymes will be used ?
What other part of DNA strand will this restriction enzyme attack ?
I'm not looking for freebies, although they are welcome. I'm looking more into where the answers can be found. If you can point me in the right direction it will be great.
first: your idea is a little weird. you can't just add restriction enzymes to cells, that just won't work - first restriction enzymes won't get into cells, and then if they do get in and make it inside the nucleus (would never happen) then you will digest all the DNA. Even if you will find a restriction enzyme that will only cut in your gene of interest, it will only be ligated back by non-homologous end-joining and/or homologous recombination.
about your questions:
1. Use a cell line derived from breast cancer. that way you'll only have breast cancer cells.
2. if you use a stabilized cell line, they should specify what gene was duplicated
3. Doesn't matter: all are equally useless.
4. all of it
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
Yes but if you take a look at "http://library.thinkquest.org/19037/therapy2.html" you will find that Thousands of varieties of restriction enzymes exist, each recognizing only a single nucleotide sequence. Once it finds that sequence in a strand of DNA, it attacks it and splits the base pairs apart, leaving single helix strands at the end of two double helixes. Scientists are then free to add any genetic sequences they wish into the broken chain and, afterwards, the chain is repaired (as a longer chain with the added DNA) with another enzyme called ligase. Hence, any form of genetic material can be spliced together; bacteria and chicken DNA can, and have been, combined. Thank you for the information, and i welcome any further criticism that you can see.
ok....but restriction enzymes aren't used on living cells. living cells have repair mechanisms that repair breaks and proteins that nullify these enzymes before they do any damage. usually restriction enzymes are used on naked dna like plasmids.
If you look closely at the article, gene therapy uses many methods, the plasmid method was only mentioned for use with bacteria. To infect human cells is a much harder problem, usually you'd have to manufacture some sort of virus to carry your gene of interest into the cell, and that is PHD level work.
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That's definitelly true, but this sequence is usually like 4, 6 or 8 nucleotides long, so you can calculate by yourself, how many posibilities there are with 4 nucleotides (A,C,G and T) and how many times just by chance will you cut the human 3 Gbps genome
Cis or trans? That's what matters.
This is a bit to sophomoric for even a science fair project.
the questions you ask reveal that you know only the terms and have no idea how to run the experiment, much less obtaining and culturing breast cancer cells.
Try to find an experimental protcol that has substance and can be understood and accmplished within your acquired understanding, capabilties and facilities.
6 posts • Page 1 of 1
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