Login

Join for Free!
118859 members


antibody staining

Discussion of all aspects of cellular structure, physiology and communication.

Moderator: BioTeam

antibody staining

Postby gael » Tue Nov 03, 2009 10:33 am

Hi,
I'm doing immunofluorescence in vitro and in vivo, and i found a new subcellular location for a protein with one antibody (which has been use in many publication). Pre-incubation with the immunogen peptide (blocking peptide) result in the loss of the signal. Overexpression of this protein in cells can be also seen. But using an other antibody shows a different pattern. Is it possible that one antibody (polyclonal) recognize two different proteins (preincubation with the peptide reveal the high affinity for my protein)?
Thanks for help.
g
gael
Garter
Garter
 
Posts: 1
Joined: Tue Nov 03, 2009 10:24 am

Postby JackBean » Tue Nov 03, 2009 11:13 am

Sure, polyclonal antibody can recognise like everything...
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.
User avatar
JackBean
Inland Taipan
Inland Taipan
 
Posts: 5689
Joined: Mon Sep 14, 2009 7:12 pm

Postby biohazard » Tue Nov 03, 2009 1:27 pm

Well, depends on the context.

In immunology, when talking about poly- vs. monoclonal antibodies, the specificity of the antibody can be the same antigen, just the polyclonal antibody recognizes many different epitopes and monoclonal just one.

Polyclonal antibodies against a single antigen can be produced in animals and they were the main antibodies used in immunology before hybridoma technology allowed the production of epitope-specific monoclonal antibodies.

Even today, polyclonal antibodies are sometimes used, for example when it is more important to bind the antigen (e.g. if you want to neutralize/eliminate it) than is to obtain an ultra-specific outocome.
User avatar
biohazard
King Cobra
King Cobra
 
Posts: 776
Joined: Sun Apr 08, 2007 6:45 pm



Return to Cell Biology

Who is online

Users browsing this forum: No registered users and 5 guests