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Freezing Het-1A cellsModerator: BioTeam
2 posts • Page 1 of 1
Freezing Het-1A cellsI recently obtain via ATCC Het-1A cells. Their product info sheet says that I must use the following cryoprotectant medium: L-15 plus 10% FBS, 1% PVP, and 7.5% DMSO. I did order the specific complete growth medium BEGM kit but I want to know if I can just DMSO to this growth medium in order to freeze or if it is necessary to get the cryoprotectant medium?
in my tissue culture days i used to just add DMSO to whatever media I normally grew the cell lines, and it worked for the lines I worked with. However, i never worked with Het-1A, it may be that this particular line is more sensitive
"I have no intention of stopping anytime soon. I want to understand the universe and answer the big questions, that is what keeps me going" - Stephen Hawking
2 posts • Page 1 of 1
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