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Freezing Het-1A cells

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Freezing Het-1A cells

Postby lrarias » Mon Oct 26, 2009 5:40 pm

I recently obtain via ATCC Het-1A cells. Their product info sheet says that I must use the following cryoprotectant medium: L-15 plus 10% FBS, 1% PVP, and 7.5% DMSO. I did order the specific complete growth medium BEGM kit but I want to know if I can just DMSO to this growth medium in order to freeze or if it is necessary to get the cryoprotectant medium?
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Postby MrMistery » Fri Oct 30, 2009 2:31 pm

in my tissue culture days i used to just add DMSO to whatever media I normally grew the cell lines, and it worked for the lines I worked with. However, i never worked with Het-1A, it may be that this particular line is more sensitive
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