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Dilution

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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Dilution

Postby carbonp » Fri Oct 16, 2009 5:37 pm

Take 5 small glass test tubes (1-5) and put 1200 μl of distilled water in each.
Now add 300 μl of 10 μM atropine to Tube 1, mix well and transfer 300 μl to Tube 2.
Mix well and transfer 300 μl to Tube 3.
Repeat up to Tube 5.
Calculate the atropine concentration in each tube.

my question is
For first test tube,
C1 = 10 micromolar
V1 = 300 microliter
C2 = we are finding
V2 = 1200 or 1500 microliter?????????????????
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Postby mith » Fri Oct 16, 2009 9:02 pm

you started with 1200, and you added 300ul fluid. for the purpose of the calculation you have 1500, however by distributing the mixed contents, your final volume in the tube is 1200.
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