bacteriophages

[taata]

Draw fully labelled diagram, and write some explanatory notes, explaining how you would quantify the number of bacteriophages in an unknown suspension.

[mothorc]

you must have 2 things:
+a TEM microscopic to have a view
+a solution of micro marbles that you know the concentration(y)
Then disolve them with an equal volume and count on microscopic.(see a phage, b micromarble)
Finally solve the function x=ab/y .
I will find the name of this method for you.

[canalon]

you must have 2 things:
+a TEM microscopic to have a view
+a solution of micro marbles that you know the concentration(y)
Then disolve them with an equal volume and count on microscopic.(see a phage, b micromarble)
Finally solve the function x=ab/y .
I will find the name of this method for you.

There are easier solutions, that do not need any microscope: search for plaque assay? counting.

[mothorc]

terible sorry
I forget the easiest
but what will you do if your virus don't have plaque (special animal or plant virus)

[canalon]

terible sorry
I forget the easiest
but what will you do if your virus don't have plaque (special animal or plant virus)

First, related to the question at the beginning of the thread, I do not think you have to take this into account: He's talking about bacteriophage, You just a sensitive strain of bacteria, the one used for propagating the phage would be good
Then if you cannot use plaque, your method seem good to me, although a real pain to prepare compared to the other...

Patrick

[mothorc]

ok ok I see