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A gene mutation = knockdown or knockout

Genetics as it applies to evolution, molecular biology, and medical aspects.

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A gene mutation = knockdown or knockout

Postby steelcat » Sat Sep 05, 2009 12:57 am

A gene mutation = knockdown or knockout or both? :P :P 8) :P
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Postby canalon » Sat Sep 05, 2009 2:05 am

Both or neither or something else, but not both at the same time...
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Postby jonmoulton » Mon Sep 14, 2009 7:52 pm

Where a gene is altered to lose its function, this is a knockout.

Where a gene is altered to decrease its expression, this is a knockdown.

Knockdown is also used to describe RNA-level manipulations to decrease gene expression, such as siRNA or antisense treatments. Unlike gene modifications, treatments with oligos have temporary effects. However, stable expression of antisense or siRNA from a plasmid (or from stably integrated DNA) can have long-term effects.
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