Help: PPAR purification

[janciey227]

Hello,
I am doing PPARs (a nuclear receptor, a nuclear protein) purification by using PPARs transformed E.coli.
But by now I have not got any PPARs. I do not know why.
If there is anyone who knows about this or has done some experiments about this, please help me.
Tell me something about PPARs purification, or give me some advice.
Thank you very much.