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northern blot analysis

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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northern blot analysis

Postby proteinz » Sat Aug 01, 2009 12:14 pm

hi guys! i am having a hair pulling moment here i cant determine what my northern blot results mean i hope someone can help...
i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting two transcripts at approx 900bp and 1200bp what does this mean?
thanks in advance
:D
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Postby MrMistery » Sat Aug 01, 2009 3:10 pm

maybe unprocessed and processed RNA?
I have never used northern blots (honestly I didn't know anyone still used them) so I'm just taking a guess here
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
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Re: northern blot analysis

Postby robsabba » Thu Aug 27, 2009 7:25 pm

proteinz wrote:hi guys! i am having a hair pulling moment here i cant determine what my northern blot results mean i hope someone can help...
i ran c. elegans RNA and detected with cDNA probe. the length of the (cdna) gene is 594bp. my gDNA sequence is approx 900bp . i am getting two transcripts at approx 900bp and 1200bp what does this mean?
thanks in advance
:D

Is it possible you are picking up a mRNA from a similar gene that produces a larger transcript?
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