maping and linkage problems

[hemu0912]

if the E. coli genome is 4 X 106 bp, answer the following questions.?
1.If the E. coli genome is 4 X 106 bp, answer the following questions.
a. If this circular molecule was made linear, how long would it be in
cm?
b. In a single E. coli cell, what is the weight (in μgrams) of this DNA?
c. If you extracted chromosomal DNA from a culture of E. coli and had
200 μgrams, how many moles would you have of this molecule?

2.Knowing the genome size of E. coli and the fact that it can divide ca.
every 20 minutes (under optimal growth conditions), calculate the
rate of DNA replication in bp/sec (assume only one origin of
replication and bidirectional replication).

3. Explain how and why the following circumstances influence
characteristics of temperature-induced DNA melting.
a. Percentage of GC base pairs
b. Urea (forms hydrogen bonds with bases)
c. Sodium chloride (neutralizes negatively charged phosphates)

Friends please help me out. i dont get any of those answers.
Thanks

[hemu0912]

NO REPLY YET.......

[canalon]

Read the rules
Do your own homework.

[reyhane]

1.

10 bp = 3.4 nanometers

4 X 106 bp / 0.34 nanometers = 1.36 x 106 nanometers = 1.36 x 10-1 cm !!